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The yeast two-hybrid system/ edited by Paul L. Bartel and Stanley Fields.

Contributor(s): edited by Bartel, Paul L | Fields, StanleyMaterial type: Text

TextPublication details: New York: Oxford University Press, 1997Edition: 1st edDescription: xi, 344p.: ill.; 24 cmSubject(s): Protein Binding -- Research -- Methodology | Yeast Fungi | PlasmidsDDC classification: 574.87322

Contents:

1 The Two-Hybrid System: a personal view Part I The Two-Hybrid Screen 2 Searching for Interacting Proteins with the Two-Hybrid System I 3 Searching for Interacting Proteins with the Two-Hybrid System II 4 Searching for Interacting Proteins with the Two-Hybrid System III 5 Constructing an Activation Domain-Fusion Library Part II Analyzing Interaction with the Two-Hybrid System 6 Obtaining Structural Information about Protein Complexes with the Two-Hybrid System 7 The Reverse Two-Hybrid System 8 Dissecting the Biological Relevance of Multiple Interactions 9 Investigation of Ligand/Receptor Interactions and the Formation of Tertiary Complexes 10 Two-Hybrid Analysis of Protein-Protein Interactions in the Yeast Pheromone Response Pathway 11 Two-Hybrid Screening and the Cell Cycle 12 Two-Hybrid Analysis of Genetic Regulatory Networks Part III Variations on the Two-Hybrid Theme 13 Mammalian Cells as Hosts for Two-Hybrid Studies of Protein-Protein Interaction 14 Detection of Protein-Protein Interactions Dependent on Post-Translational Modification 15 Protein Interactions Mediated by Small Molecule Ligands 16 Use of a Combinatorial Peptide Library in the Two-Hybrid Assay Part IV Related Methodologies 17 Isolation of DNA-Binding Proteins using a One-Hybrid Genetic Screen 18 A Three-Hybrid System to Detect and Analyze RNA-Protein Interactions in Vivo 19 Split Ubiquitin: a Sensor of Protein Interactions in Vivo

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Holdings
Item type	Current library	Call number	Status	Notes	Date due	Barcode	Item holds
General Books Science Library	Science Library, Sikkim University Science Library General Section	574.87322 BAR/T (Browse shelf(Opens below))	Available	Books For SU Science Library		P03102

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1 The Two-Hybrid System: a personal view

Part I The Two-Hybrid Screen
2 Searching for Interacting Proteins with the Two-Hybrid System I
3 Searching for Interacting Proteins with the Two-Hybrid System II
4 Searching for Interacting Proteins with the Two-Hybrid System III
5 Constructing an Activation Domain-Fusion Library

Part II Analyzing Interaction with the Two-Hybrid System
6 Obtaining Structural Information about Protein Complexes with the Two-Hybrid System
7 The Reverse Two-Hybrid System
8 Dissecting the Biological Relevance of Multiple Interactions
9 Investigation of Ligand/Receptor Interactions and the Formation of Tertiary Complexes
10 Two-Hybrid Analysis of Protein-Protein Interactions in the Yeast Pheromone Response Pathway
11 Two-Hybrid Screening and the Cell Cycle
12 Two-Hybrid Analysis of Genetic Regulatory Networks

Part III Variations on the Two-Hybrid Theme
13 Mammalian Cells as Hosts for Two-Hybrid Studies of Protein-Protein Interaction
14 Detection of Protein-Protein Interactions Dependent on Post-Translational Modification
15 Protein Interactions Mediated by Small Molecule Ligands
16 Use of a Combinatorial Peptide Library in the Two-Hybrid Assay

Part IV Related Methodologies
17 Isolation of DNA-Binding Proteins using a One-Hybrid Genetic Screen
18 A Three-Hybrid System to Detect and Analyze RNA-Protein Interactions in Vivo
19 Split Ubiquitin: a Sensor of Protein Interactions in Vivo

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