Normal view MARC view ISBD view

The yeast two-hybrid system/ edited by Paul L. Bartel and Stanley Fields. - 1st ed. - New York: Oxford University Press, 1997. - xi, 344p.: ill.; 24 cm.

1 The Two-Hybrid System: a personal view

Part I The Two-Hybrid Screen
2 Searching for Interacting Proteins with the Two-Hybrid System I
3 Searching for Interacting Proteins with the Two-Hybrid System II
4 Searching for Interacting Proteins with the Two-Hybrid System III
5 Constructing an Activation Domain-Fusion Library

Part II Analyzing Interaction with the Two-Hybrid System
6 Obtaining Structural Information about Protein Complexes with the Two-Hybrid System
7 The Reverse Two-Hybrid System
8 Dissecting the Biological Relevance of Multiple Interactions
9 Investigation of Ligand/Receptor Interactions and the Formation of Tertiary Complexes
10 Two-Hybrid Analysis of Protein-Protein Interactions in the Yeast Pheromone Response Pathway
11 Two-Hybrid Screening and the Cell Cycle
12 Two-Hybrid Analysis of Genetic Regulatory Networks

Part III Variations on the Two-Hybrid Theme
13 Mammalian Cells as Hosts for Two-Hybrid Studies of Protein-Protein Interaction
14 Detection of Protein-Protein Interactions Dependent on Post-Translational Modification
15 Protein Interactions Mediated by Small Molecule Ligands
16 Use of a Combinatorial Peptide Library in the Two-Hybrid Assay

Part IV Related Methodologies
17 Isolation of DNA-Binding Proteins using a One-Hybrid Genetic Screen
18 A Three-Hybrid System to Detect and Analyze RNA-Protein Interactions in Vivo
19 Split Ubiquitin: a Sensor of Protein Interactions in Vivo

Subjects--Topical Terms:
Protein Binding--Research--Methodology
Yeast Fungi
Plasmids

Dewey Class. No.: 574.87322 / BAR/T