Comprehensive biophysics: biophysics techniques for structural characterization of macromolecules/ edited by Edward H. Egelma

VOLUME 1 Biophysical Techniques for Structural Characterization of Macromolecules
1.1 Volume Introduction HJ Dyson 1
Overview and Historical
1.2 Cantor and Schimmel - 30 Years Later C Cantor and P Schimmel 2
Protein Production Strategies for High-Throughput Structure Determination
1.3 Efficient Strategies for Production of Eukaryotic Proteins JD Cossar and CHArrowsmith 4
X-ray Crystallography
1.4 X-ray Crystallography: Crystallization EE Abola, VCherezov, and RC Stevens 34
1.5 X-ray Crystallography; Data Collection Strategies and Resources Gonzalez 64
1.6 Phasing of X-ray Data I Uson 92
1.7 Refinement of X-ray Crystal Structures AT Bninger and PD Adams 105
1.8 Structure Validation
and Analysis M-A Elsliger and IA Wilson " 116
NMR Spectroscopy
1.9 Introduction to Solution State NMR Spectroscopy HJ Dyson and AG Palmer III 136
1.10 Solid State NMR Methods M Baldus 160
1.11 The Hybrid Solution/Solid-State NMR Method for Membrane Protein Structure
Determination G Veglia, N} Traaseth, L Shi, R Verardi, T Gopinath, and M Gustavsson 182
1.12 Labeling Techniques M Takeda and M Kainosho 199
1.13
NMR Spectroscopy: NMR Relaxation Methods AG Palmer III 216
Electron Microscopy
1.14 Structure Determination of Macromolecular Complexes by Cryo-Electron Microscopy In vitro
and in situ F Forster, E Villfi, D Thomas, A Korinek, and W Baumeister 245
1.15 Analysis of 2-D Crystals of Membrane Proteins by Electron Microscopy Abeyrathne,
MArheit, F Kebbel, D Castano-Diez, KN Goldie, M Chami, H Stahlberg, L Renault, and
W Kuhlbrandt 277
vi Contents
1.16 Cryo-Electron Microscopy and Tomography of Virus Particles RH Rochat and WChiu 311
Mass Spectrometry
1.17 Mass Spectrometry A Nakorchevsky and JR Yates 341
SAXS
1.18 Small and Wide Angle X-ray Scattering from Biological Macromolecules and their Complexes
in Solution S Doniach and J Lipfert 376
Ultrafast Spectroscopic Techniques
1.19 Ultrafast Structural Dynamics of Biological Systems M Chergui 398
EPR and Other Electron Spectroscopies
1.20 Electron Magnetic Resonance CJ Bender 425
Computation
1.21 Computation of Structure, Dynamics, and Thermodynamics of Proteins S Otdziej,
C Czaplewski, A Liwo, }A Vila, and HA Scheraga 494
Fast Flow
1.22 Rapid Mixing Techniques for the Study of Enzyme Catalysis S Mitic and S de Vries 514
Other Spectroscopy - UV-VIs, CD, Raman, VIbrational CD Applied in Biophysical Research
1.23 Optical Spectroscopy RSchweitzer-Stenner and JB Sojfer 533
FRET/Fluorescence
1.24 Fluorescence and FRET: Theoretical Concepts 101 RM Clegg 592