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<br/>VOLUME 1 Biophysical Techniques for Structural Characterization of Macromolecules<br/>1.1 Volume Introduction HJ Dyson 1<br/>Overview and Historical<br/>1.2 Cantor and Schimmel - 30 Years Later C Cantor and P Schimmel 2<br/>Protein Production Strategies for High-Throughput Structure Determination<br/>1.3 Efficient Strategies for Production of Eukaryotic Proteins JD Cossar and CHArrowsmith 4<br/>X-ray Crystallography<br/>1.4 X-ray Crystallography: Crystallization EE Abola, VCherezov, and RC Stevens 34<br/>1.5 X-ray Crystallography; Data Collection Strategies and Resources Gonzalez 64<br/>1.6 Phasing of X-ray Data I Uson 92<br/>1.7 Refinement of X-ray Crystal Structures AT Bninger and PD Adams 105<br/>1.8 Structure Validation<br/>and Analysis M-A Elsliger and IA Wilson " 116<br/>NMR Spectroscopy<br/>1.9 Introduction to Solution State NMR Spectroscopy HJ Dyson and AG Palmer III 136<br/>1.10 Solid State NMR Methods M Baldus 160<br/>1.11 The Hybrid Solution/Solid-State NMR Method for Membrane Protein Structure<br/>Determination G Veglia, N} Traaseth, L Shi, R Verardi, T Gopinath, and M Gustavsson 182<br/>1.12 Labeling Techniques M Takeda and M Kainosho 199<br/>1.13<br/>NMR Spectroscopy: NMR Relaxation Methods AG Palmer III 216<br/>Electron Microscopy<br/>1.14 Structure Determination of Macromolecular Complexes by Cryo-Electron Microscopy In vitro<br/>and in situ F Forster, E Villfi, D Thomas, A Korinek, and W Baumeister 245<br/>1.15 Analysis of 2-D Crystals of Membrane Proteins by Electron Microscopy Abeyrathne,<br/>MArheit, F Kebbel, D Castano-Diez, KN Goldie, M Chami, H Stahlberg, L Renault, and<br/>W Kuhlbrandt 277<br/>vi Contents<br/>1.16 Cryo-Electron Microscopy and Tomography of Virus Particles RH Rochat and WChiu 311<br/>Mass Spectrometry<br/>1.17 Mass Spectrometry A Nakorchevsky and JR Yates 341<br/>SAXS<br/>1.18 Small and Wide Angle X-ray Scattering from Biological Macromolecules and their Complexes<br/>in Solution S Doniach and J Lipfert 376<br/>Ultrafast Spectroscopic Techniques<br/>1.19 Ultrafast Structural Dynamics of Biological Systems M Chergui 398<br/>EPR and Other Electron Spectroscopies<br/>1.20 Electron Magnetic Resonance CJ Bender 425<br/>Computation<br/>1.21 Computation of Structure, Dynamics, and Thermodynamics of Proteins S Otdziej,<br/>C Czaplewski, A Liwo, }A Vila, and HA Scheraga 494<br/>Fast Flow<br/>1.22 Rapid Mixing Techniques for the Study of Enzyme Catalysis S Mitic and S de Vries 514<br/>Other Spectroscopy - UV-VIs, CD, Raman, VIbrational CD Applied in Biophysical Research<br/>1.23 Optical Spectroscopy RSchweitzer-Stenner and JB Sojfer 533<br/>FRET/Fluorescence<br/>1.24 Fluorescence and FRET: Theoretical Concepts 101 RM Clegg 592<br/> |
