000 01284cam a2200301 a 4500
999 _c169530
_d169530
020 _a9780387312781
020 _a0387312781
040 _cCUS
082 0 0 _a543.56
_bLAK/P
100 1 _aLakowicz, Joseph R.
245 1 0 _aPrinciples of fluorescence spectroscopy/
_cJoseph R. Lakowicz.
250 _a3rd ed.
260 _aNew York :
_bSpringer,
_cc2006.
300 _axxvi, 954 p. :
_bill. ;
_c29 cm. +
_e1 CD-ROM (4 3/4 in.)
504 _aIncludes bibliographical references and index.
505 _aIntroduction to Fluorescence -- Instrumentation for Fluorecence Spectroscopy -- Fluorophores -- Time-Domain Lifetime Measurements -- Frequency-Domain Lifetime Measurements -- Solvent and Environmental Effects -- Dynamics of Solvent and Spectral Relaxation -- Quenching of Fluorescence -- Mechanisms and Dynamics of Fluorescence Quenching -- Fluorescence Anisotropy -- Time-Dependent Anisotropy Decays -- Advanced Anisotropy Concepts -- Energy Transfer -- Time Resolved Energy Transfer and Conformational Distributions of Biopolymers -- Energy Transfer to Multiple Acceptors, in One-, Two- or Three-Dimensions -- Protein Fluorescence -- Time-Resolved Protein Fluorescence -- Multiphoton Excitation and Microscopy -- Fluorescence Sensing -- Novel Fluorophobes -- DNA Technology -- Fluorescence Lifetime Imaging Microscopy -- Single-Molecule Detection -- Fluorescence Correlation Spectroscopy -- Radiative Decay Engineering: Metal-Enhanced Fluorescence -- Radiative Decay Engineering: Surface Plasmon-Coupled Emission -- Appendix I. Corrected Emission Spectra -- Appendix II. Fluorescent Lifetime Standards -- Appendix III. Additional Reading -- Answers to Problems -- Index.
650 0 _aFluorescence spectroscopy.
650 0 _aAnalytical biochemistry.
650 0 _aBiochemistry.
650 0 _aBiotechnology.
942 _cSC79