000			01449nam a2200193Ia 4500
999			_c148398 _d148398
040			_cCUS
082			_a572.8 _bSAM/T
100			_aSambrook, Joseph _921075
245		4	_aThe condensed protocols: from molecular cloning: a laboratory manual/ _cJoseph Sambrook, David W. Russell.
250			_a1st ed.
260			_aNew York: _bCold Spring Harbor Laboratory Press, _c2006.
300			_av, 800p. _c28 cm.
505			_a1 Plasmids and their usefulness in molecular cloning 2 Bacteriophage and its vectors 3 Working with bacteriophage M13 vectors 4 Working with high-capacity vectors 5 Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis 6 Preparation and analysis of eukaryotic genomic DNA 7 Extraction, purification, and analysis of mRNA from eukaryotic cells 8 In vitro amplification of DNA by the polymerase chain reaction 9 Preparation of radiolabeled DNA by and RNA probes 10 Working with Synthetic oligonucleotide probes 11 Preparation of cDNA libraries and gene identification 12 DNA sequencing 13 Mutagenesis 14 Screening expression libraries 15 Expression of cloned genes in escherichia coli 16 Introducing cloned genes into cultured mammalian cells 17 Analysis of gene expression in mammalian cells 18 Protein interaction technologies
650			_aMolecular Cloning _921076
650			_aBiological Techniques _921077
650			_aMolecular Biology _912098
700			_aRussell, David W. _921078
942			_cSC79 _02