TY - BOOK TI - Human stem cell manual : a laboratory guide SN - 9780123704658 U1 - 616.02774 PY - 2007/// CY - Amsterdam, Boston PB - Elsevier/Academic Press KW - Biology KW - Research KW - Methodology KW - Embryonic stem cells KW - Stem cells N1 - Part I Basic Methods in Stem Cell Culture Human Embryonic Stem Cell Culture Rodolfo Gonzalez, Robin L. Wesselschmidt, Philip H. Schwartz, and Jeanne f. Loring Human Feeder Cells, Feeder-free, and Defined Culture Systems Robin L. Wesselschmidt and Jeanne E Loring Mouse Embryonic Fibroblast Feeder Cells Chris Stubban and Robin L. Wesselschmidt Cryopreservation of Human Embryonic Stem Cells Chris Stubban, Robin L. Wesselschmidt, Igor Katkov, and Jeanne F. Loring Part II Characterization of Stem Cells Classical Cytogenetics: Karyotyping Robin L. Wesselschmidt and Jeanne F. Loring Spectral Karyotyping and Fluorescent in situ Hybridization Suzanne Peterson, Stevens Rehen, Willem Westra, Yun Yung, and Jerold Chun Genotype and Epigenotype by Single Nucleotide Polymorphism (SNP) Analysis Kevin Cunderson, David L. Barker, Marina Bibikova, and Jian-Bing Fan Flow Cytometric Analysis of Human Embryonic Stem Cells Andrew L. Laslett, Andrew Fryga, and Martin F. Pera ER -