Determination of bacteria diversity and its antibiotic resistance in gradient natural thermal fields spanning across thermophilic to psychrophilic environments/
- Gangtok, Sikkim : Sikkim University, 2022.
- 72p.: ill. ; 29 cm.
Introduction 3-7 Aims and objectives 8 Review Literature 9-20 3.1. A view of different Natural thermal zones 3.2. The impacts of antibiotic resistance on human health and the environment Methodology 21-27 4.1. Sampling and site description4.2. Culture Dependent Methods 4.2.1. Isolation and characterization of bacterial isolates 4.2.2. Identification of bacterial isolates and Phylogeny 4.2.3. Antibiotic Susceptibility Testing of the bacterial isolates4.3. Culture Independent Methods 4.3.1. Elemental analysis by Inductively Coupled Plasma Mass Spectrometry 4.3.2. Metagenomic DNA extraction 4.3.3. Shotgun Metagenomic Sequencing 4.3.4. Metagenomic data analysis 4.3.5. Antibiotic Resistant Gene detection from Metagenomic sequences 4.3.6. Statistical Analysis Results 28-51 Discussion 52-60 Conclusion 61 References 62-72