Unit 1: Principle NMR and pulsed NMR Introduction -- Spin Magnetic properties -- Resonance condition -- Larmor Frequency -- NMR instrumentation -- comparison of NMR & EPR -- chemical shift -- spin-spin coupling -- Coupling constant -- first and second order spectra -- simplification of complex spectra -- Boltzmann Statistics -- Pulsed NMR -- Basic principle of FT technique -- Relaxation process -- Bloch equations -- Relaxation times -- Line shape and line width analysis
Unit 2: 1H and 13C NMR spectroscopy Interpretation of 1H NMR -- assignment of signals -- influence of factors on chemical shift of protons -- 13C NMR -- spin decoupling -- double resonance -- Nuclear Overhauser Effect -- Off Resonance Decoupling -- CIDNP -- 13C assignment of signals -- additivity rule -- calculation of chemical shifts for aromatic and aliphatic compounds -- 2D NMR -- DEPT 13C -- 13C correlation COSY, HETCOR, NOE & NOSEY- multidimensional NMR
Unit 3: Applications of NMR Applications of 31P, 19F and 15N -- NMR spectroscopy used in structural problem -- NMR of fluxional molecules -- evaluation of rate constants -- solid state NMR -- magic angle spinning -- NMR of paramagnetic molecules -- contact shifts and shift reagents -- NMR imaging -- contrast agents
Unit 4: EPR Spectroscopy Principle of EPR -- instrumentation -- total spin Hamiltonian -- presentation of the spectrum -- hyperfine splitting -- super hyperfine structure -- EPR of hydrogen atom -- splitting in isotropic systems involving more than one nucleus -- EPR spectra of free radicals in solution -- methyl radical, benzene anion, p-benzosemiquinone radical anion, p- nitrobenzoate dianion and naphthalene anion -- Evaluation of g and A tensors -- factors affecting the magnitude of g values -- anisotropy -- zero field splitting -- Kramer's theory & degeneracy -- triplet EPR
Unit 5: Application and advanced EPR Application of EPR in transition metal complexes VO2+ Fe3+ Co2+, Mn2+, Ni2+ and bis-salicylaldimine copper (II) -- Jahn-Teller theory & distortion studies in Cu(II) complexes -- evaluation of spin -- orbital coupling constant -- exchange coupled EPR -- multi resonance EPR -- cw and pulsed EPR and ENDOR -- MEMS and DAVIS -- ESEEM -- HYSCORE -- ESR Spin trapping -- spin labeling -- EPR imaging