Molecular Biology and Genetic Engineering/
P.K. Gupta.
- 1st ed.
- New Delhi: Rastogi Publications, 2011.
- xxi, 590p. 26 cm.
Part I Molecular Biology 1 Molecular Biology and Genetic Engineering : Definition, History and Scope 2 Chemistry of the Cell : 1. Micromolecules (Sugars, Fatty Acids, Amino Acids, Nucleotides and Lipids) 3 Chemistry of the Cell : 2. Macromolecules (Nucleic Acids, Proteins and Polysaccharides) 4 Chemistry of the Gene : Synthesis, Modification and Repair of DNA 5 Organization of Genetic Material : 1. Packaging of DNA as Nucleosomes in Eukaryotes 6 Organization of Genetic Material : 2. Repetitive and Unique DNA Sequences 7 Organization of Genetic Material : 3. Split Genes, Overlapping Genes, Pseudogenes and Cryptic genes 8 Multigene Families in Eukaryotes 9 Organization of Mitochondrial and Chloroplast Genomes 10 The Genetic Code 11 Protein Synthesis Apparatus : Ribosome, Transfer RNA and Aminoacyl -tRNS Synthetases 12 Expression of Gene : Protein Synthesis 1. Transcription in Prokaryotes and Eukaryotes 13 Expression of Gene : Protein Synthesis 2. RNA Processing (RNA Splicing, RNA Editing and Ribozymes) 14 Expression of Gene : Protein Synthesis 3. Synthesis and Transport of Proteins (Prokaryotes and Eukaryotes) 15 Regulation of Gene Expression 1. Operon Circuits in Bacteria and other Prokaryotes 16 Regulation of Gene Expression 2. Circuits for Lytic Cycle and Lysogeny in Bacteriophages 17 Regulation of Gene Expression 3. A Variety of Mechanisms in Eukaryotes (Including Cell Receptors and Cell Signalling)
PART II Genetic Engineering 18 Recombinant DNA and Gene Cloning 1. Cloning and Expression Vectors 19 Recombinant DNA and Gene Cloning 2. Chimeric DNA, Molecular Probes and Gene Libraries 20 Polymerase Chain Reaction (PCR) and Gene Amplification 21 Isolation, Sequencing and Synthesis of Genes 22 Proteins : Separation, Purification and Identification 23 Immunotechnology 1. B-Cells, Antibodies, Interferons and Vaccines 24 Immunotechnology 2. T -Cell Receptors and MHC Restriction 25 Immunotechnology 3. Hybridoma and Monoclonal Antibodies (mAbs) 26 Transfection Methods and Transgenic Animals 27 Animal and Human Genomics : Molecular Maps and Genome Sequences 28 Biotechnology In Medicine : 1. Vaccines, Diagnostics and Forensics 29 Biotechnology in Medicine : 2. Gene Therapy 30 Biotechnology in Medicine : 3. Pharmacogenetics / Pharmacogenomics and Personalized Medicine 31 Plant Cell and Tissue Culture : Production and Uses of Haploids 32 Gene Transfer Methods In Plants 33 Transgenic Plants : Genetically Modified (GM) Crops and Floricultural Plants 34 Plant Genomics : Molecular Maps and Genome Sequences 35 Genetically Engineered Microbes (GEMs) and Microbial Genomics