DNA Protein Interactions: principles and protocols/ 
edited by Tom Moss and Benoit Leblanc. 
 - 3rd ed. 
 - New York:  Humana Press,  2009. 
 - xvii, 680p.:  ill.;  28 cm. 
<br/><br/>1. Filter-Binding Assays<br/>2. Electrophoretic Mobility Shift Assays for the Analysis of DNA-Protein Interactions<br/>3. DNase I Footprinting <br/>4. Exonuclease III Footprinting on Immobilized DNA Templates 5. Hydroxyl Radical Footprinting of Protein-DNA Complexes<br/>6. The Use of Diethyl Pyrocarbonate (DEPC) and Potassium Permanganate as Probes for Strand Separation and Structural Distortions in DNA<br/>7. Uranyl Photofootprinting<br/>8. Identification of Protein/DNA Contacts with Dimethyl Sulfate: Methylation Protection and Methylation Interference<br/>9. Ethylation Interference Footprinting of DNA-Protein Complexes<br/>10. Site-Directed Cleavage of DNA by Protein-Fe(II) EDTA Conjugates within Model Chromatin Complexes<br/>11. Identification of Nucleic Acid High Affinity Binding Sequences of Proteins by SELEX<br/>12. Identification of Sequence-Specific DNA-Binding Proteins by Southwestern Blotting<br/>13. Footprinting DNA-Protein Interactions in Native Polyacrylamide Gels by Chemical Nucleolytic Activity of 1,10-Phenanthroline-Copper<br/>14. Determination of a Transcription Factor-Binding Site by Nuclease Protection Footprinting onto Southwestern Blots<br/>15. Use of a Reporter Gene Assay in Yeast for Genetic Analysis of DNA-Protein Interactions<br/>16. Chromatin Immunoprecipitation in Mammalian Cells<br/>17. Sequential Chromatin Immunoprecipitation Protocol: ChIP-reChIP <br/>18. Profiling Genome-Wide Histone Modifications and Variants by ChIP-chip on Tiling Microarrays in S. cerevisiae<br/>19. Nucleosome Mapping<br/>20. In Cellulo DNA Analysis (LMPCR Footprinting)<br/>21. Atomic Force Microscopy Imaging and Probing of DNA, Proteins and Protein-DNA Complexes: Silatrane Surface Chemistry<br/>22. Two-Dimensional Crystallisation of Soluble Protein Complexes<br/>23. Assays for Transcription Factor Activity<br/>24. Ultraviolet Crosslinking of DNA-Protein Complexes via 8-Azidoadenine<br/>25. Static and Kinetic Site-Specific Protein-DNA Photocrosslinking: Analysis of Bacterial Transcription Initiation Complexes<br/>26. Use of Site-Specific Protein-DNA Photocrosslinking of Purified Complexes to Analyze the Topology of the RNA Polymerase II Transcription Initiation Complex<br/>27. Site-Directed DNA Cross-Linking of Large Multi-Subunit Protein-DNA Complexes<br/>28. Functional Studies of DNA-Protein Interactions Using FRET Techniques<br/>29. Single-Molecule FRET Analysis of Protein-DNA<br/>30. Analysis of DNA Supercoiling Induced by DNA-Protein Interactions<br/>31. The Cruciform DNA Mobility Shift Assay: A Tool to Study Proteins that Recognize Bent DNA<br/>32. Plasmid Vectors for the Analysis of Protein Induced DNA-Bending<br/>33. Analysis of Distant Communication on Defined Chromatin Templates In Vitro<br/>34. A Competition Assay for DNA Binding Using the Fluorescent Probe ANS<br/>35. Fluorescence Spectroscopy and Anisotropy in the Analysis of DNA-Protein Interactions<br/>36. Circular Dichroism for the Analysis of Protein-DNA Interactions<br/>37. Defining the Thermodynamics of Protein/DNA Complexes and Their Components Using Micro-Calorimetry<br/>38. Surface Plasmon Resonance Assays of DNA-Protein Interactions  
<br/><br/><label>ISBN: </label>9781603270144 
<br/><br/><label>Subjects--Topical Terms: </label><br/>DNA-Protein Interactions<br/>Human Genetics
<br/><br/><label>Dewey Class. No.: </label>572.86  / MOS/D