Molecular biology and biotechnology: Microbial methods/
Manoj V. Parakhia [et al.].
- 1st ed.
- New Delhi: NIPA, 2010.
- xix, 360p.: ill.; 25 cm.
1. Laboratory safety rules 2. Milestones in DNA history and biotechnology 3. Contribution in development of DNA and biotechnology 4. Equipments required in a molecular marker laboratory 5. Basic calculations used in microbial biotechnology 6. Basic principals in preparation of reagents required in microbial biotechnology 7. Isolation of bacterial genomic DNA 8. Isolation of RNA from yeast 9. Nucleotide composition of RNA 10. DNA melting point determination 11. Quantitative determination of DNA by DPA method and spectroscopic method 12. Estimation of RNA by Orcinol method 13. Electrophoresis 14. SDS-PAGE electrophoresis 15. Polymerase chain reaction 16. Restriction enzyme digestion 17. Extraction of DNA fragments from Agarose Gel 18. Isolation the Mammalian DNA from whole blood 19. Isolation of Plasmid DNA from bacteria 20. Random Amplified Polymorphic DNA RAPD 21. Amplified Fragment Leanth Polymorphism AFLP 22. Southern blotting 23. Ligation of insert DNA to Vector DNA 24. Bacterial transformation 25. Detection of plasmid by transformation 26. Conjugation in E.Coli by plate method 27. Isolation of Lactose non fermentor mutants of E.Coli by Physical mutagenesis 28. Isolation of auxotrophic mutant by chemical 5 Bu mutagenesis 29. Mutagenesis direct selection 30. Mutagenesis indirect selection 31. Agrobacterium mediated gene transfer from bacteria to plant. 32. Western blotting 33. Northern blotting 34. PCR-sequencing 35. Overview of metagenomics 36. Calculation of similarity index values and construction of Dendrogram using NTSYSpc 2.0.