Protien Phosphorylation/ A Practical Approach
edited by D.G Hardie
- 2nd
- USA: Oxford University Press, 1999.
- XXVIII, 431 Seiten : Illustrationen, Diagramme.
Preface ; 1. Study of protein phosphorylation in intact cells ; 2. Analysis of signal transduction pathways using protein kinase inhibitors and activators ; 3. Analysis of signal transduction pathways using proteinserine/threonine phosphatase inhibitors ; 4. Analysis of signal transduction pathways using molecular biological manipulations ; 5. Phosphopeptide mapping and phosphoamino acid analysis on cellulose thin-layer plates ; 6. Phosphorylation site analysis by mass spectrometry ; 7. Assay and purification of protein (serine/threonine) phosphatases ; 8. [chapter title missing] ; 9. Identification of substrates for protein-tyrosine phosphatases ; 10. Assay and purification of protein-serine/threonine kinases ; 11. Assay of protein kinases and phosphatases using specific peptide substrates ; 12. Cloning and expression of cDNAs encoding protein kinase subunits ; 13. Interaction cloning of protein kinase partners ; 14. Identifying protein kinase substrates by expression screening with solid-phase phosphorylation ; 15. Analysis of protein kinase interactions using the two-hybrid method ; 16. Analysis of protein kinase interactions using biomolecular interaction analysis ; 17. Analysis of protein kinase specificity using orientated peptide libraries ; 18. Crystallization of protein kinases and phosphatases ; Index