TY - BOOK AU - Primrose, S.B. AU - Twyman, R.M. TI - Principles of gene manipulation and genomics SN - 9788126548392 U1 - 660.65 PY - 2006/// CY - USA PB - Blackwell Publishing KW - Genetic engineering KW - Genomics KW - Gene mapping N1 - Gene manipulation in the post-genomics era -- Basic techniques -- Cutting and joining DNA molecules -- Basic biology of plasmid and phage vectors -- Cosmids, phasmids, and other advanced vectors -- Gene cloning strategies -- Sequencing genes and short stretches of DNA -- Changing genes : site-directed mutagenesis and protein engineering -- Bioinformatics -- Cloning in bacteria other than Escherichia coli -- Cloning in Saccharomyces cerevisiae and other fungi -- Gene transfer to animal cells -- Genetic manipulation of animals -- Gene transfer to plants -- Advanced transgenic technology -- The organization and structure of genomes -- Mapping and sequencing genomes -- Comparative genomics -- Large-scale mutagenesis and interference -- Analysis of the transcriptome -- Proteomics I : expression analysis and characterization of proteins -- Proteomics II : analysis of protein structures -- Proteomics III : protein interactions -- Metabolomics and global biochemical networks -- Applications of genomics : understanding the basis of polygenic disorders and identifying quantitative trait loci -- Applications of recombinant DNA technology ER -