TY  - BOOK
AU  - Mellick, Albert S., ed.
AU  - Rodgers, Linda
TI  - Lab ref: a handbook of recipes, reagents, and other reference tools for use at the bench
SN  - 9780879698157
U1  - 572.078 
PY  - 2007///
CY  - New York
PB  - Cold Spring Harbor Laboratory Press
KW  - Biochemistry
N1  - Section 1; Quantitative nucleic acid analysis: Isolation of total DNA --
Isolation of total RNA --
Selection of Poly(A) RNA from total --
Isolation and amplification of RNA from single cells and small amounts of tissue --
Hybridization analysis --
Preparation of nucleic acid (target) for array hybridization analysis --
Preparation of nucleic acid (probe) for array hybridization analysis --
Other quantitative methods --
Section 2; RNAi: RNAi in C. elegans --
RNAi in D. melanogaster --
RNAi in plants --
RNAi in mammalians --
RNAi in mouse oocytes and early embryos --
Section 3; Imaging: Preparation of embryos for live cell imaging --
Imaging fixed tissue --
Preparation of mice for imaging --
Intracellular imaging --
General information for imaging --
Section 4; Protein analysis/proteomics: Basic methods in protein analysis for proteomics --
Extraction of proteins --
Advanced protocols in identifying protein-protein interactions for proteomics --
Section 5; Arabidopsis: Microscopy --
Genetic manipulation --
Protein extraction --
Nuclear extraction --
Histochemistry of GUS fusion --
Section 6; Murine: Invitro culturing of embryonic stem cells --
Genotyping --
Immunohistochemistry of whole-mount embryos --
Other buffers and solutions --
Section 7; Storage and shipment of biological samples --
Section 8; Useful facts and tables --
Nomenclature guide --
Useful World Wide Web sites
ER  -