Lab ref: a handbook of recipes, reagents, and other reference tools for use at the bench/
edited by Albert S. Mellick and Linda Rodgers
- New York: Cold Spring Harbor Laboratory Press, 2007.
- 2v. (x, 272 p.; xi, 248 p.) : ill. ; 24 cm.
Section 1; Quantitative nucleic acid analysis: Isolation of total DNA -- Isolation of total RNA -- Selection of Poly(A) RNA from total -- Isolation and amplification of RNA from single cells and small amounts of tissue -- Hybridization analysis -- Preparation of nucleic acid (target) for array hybridization analysis -- Preparation of nucleic acid (probe) for array hybridization analysis -- Other quantitative methods -- Section 2; RNAi: RNAi in C. elegans -- RNAi in D. melanogaster -- RNAi in plants -- RNAi in mammalians -- RNAi in mouse oocytes and early embryos -- Section 3; Imaging: Preparation of embryos for live cell imaging -- Imaging fixed tissue -- Preparation of mice for imaging -- Intracellular imaging -- General information for imaging -- Section 4; Protein analysis/proteomics: Basic methods in protein analysis for proteomics -- Extraction of proteins -- Advanced protocols in identifying protein-protein interactions for proteomics -- Section 5; Arabidopsis: Microscopy -- Genetic manipulation -- Protein extraction -- Nuclear extraction -- Histochemistry of GUS fusion -- Section 6; Murine: Invitro culturing of embryonic stem cells -- Genotyping -- Immunohistochemistry of whole-mount embryos -- Other buffers and solutions -- Section 7; Storage and shipment of biological samples -- Section 8; Useful facts and tables -- Nomenclature guide -- Useful World Wide Web sites.