A comparative methods approach to the study of oocytes and embryos/
edited by Joel D. Richter
- New York: Oxford University Press, 1999.
- xv, 520 p. : ill. ; 24 cm.
- (Advances in molecular biology) .
PART ONE: METHODS FOR STUDYING MOUSE OOCYTES AND EMBRYOS -- 1. Mouse Oocyte Maturation, Fertilization, and Preimplantation Development in Vitro -- 2. Purification and Functional Analysis of Mouse Egg Zona Pellucida Glycoproteins -- 3. Methods for Analysis of Cytoplasmic Signal Transduction in Mammalian Eggs and Embryos -- 4. Intracellular Ion Measurements in Single Aggs and Embryos Using Ion-Sensitive Fluorophores -- 5. Approahces o Study Translational Control in Mouse Oocytes -- 6. Identification of Genes and Processes Guiding the Transition between the Mammalian Gamete and Embryo -- 7. Approaches for the analysis of Genomic Imprinting in Oocytes and Early Mouse Embryos -- 8. Gene Expression in Mouse Embryos: Use mRNA Differential Displays Differential Display -- 9. Use of Differential Display to Identify Gene Expression Differences in Male and Female Mouse Genital Ridges -- PART TWO: METHODS FOR STUDYING XENOPUS OOCYTES AND EMBRYOS -- 10. DNA Recombination and Repair in Xenopus Oocytes and Embryos -- 11. DNA Replication and Chromatin Assembly Using Xenopus Eggs or Embryos -- 12. Isolation and Characterization of Maked mRNPs from Xenopus Oocytes -- 13. Complicationa and Problems in the Assay of Transcription in Xenopus Oocytes, Eggs, and Embryos -- 14. Analysis of Localized RNAs in Xenopus Oocytes -- 15. InSitu Hybridization Techniques with Xenopus Embryos -- 16. Visualizing Endogenous and Exogenous Proteins in Xenopus laevis (and Other Organisms) -- 17. Qualitative Analysis of Differential Gene Expression during Early Xenopus Embryogenesis by Whole-Mount In Situ Hybridization and RT-PCR -- 18. Studying the Function of Maternal mRNAs in Xenopus Embryos: An Antisense Approach -- 19. Testing the Cell Fate Commitment of Single Blastomeres in Xenopus laevis -- PART THREE: METHODS FOR STUDYING DROSOPHILA OOCYTES AND EMBRYOS -- 20. Eggshell Assembly in Drosophila -- 21. Cell Biological and Genetic Methods for Studying Drosophila Oogenesis -- 22. Techniques for Analyzing Protein and RNA Distribution in Drosophila Ovaries and Embryos at Structural and Ultrastructural Resolution -- 23. Analysis of RNA Distribution during Drosophila Oogenesis Using Fluorescent In Situ Hybridization -- 24. Assessment of Poly(A) Tail Lengths on Specific mRNAs during Development -- 25. Three-Dimensional, Time-Lapse Microscopy of Drosophila Embryogenesis -- 26. Anatomical Techniques for Analysis of Nervous System Development in the Drosophila Embryo.