TY - BOOK AU - Primrose, Sandy AU - Twyman, Richard AU - Old, Bob TI - Principles of gene manipulation SN - 9780632059546 U1 - 575.1 PY - 2003/// CY - USA PB - Blackwell Science KW - Gene Manipulation N1 - 1 Gene manipulation: an all-embracing technique 2 Basic techniques 3 Cutting and joining DNA molecules 4 Basic biology of plasmid and phage vectors 5 Cosmids, phasmids and other advanced vectors 6 Cloning strategies 7 Sequencing and mutagenesis 8 Cloning in bacteria other than Escherichia coli 9 Cloning in Saccharomyces cerevisiae and other fungi 10 Gene transfer to animal cells 11 Genetic manipulation of animals 12 Gene transfer to plants 13 Advances in transgenic technology 14 Applications of recombinant DNA technology ER -