TY - BOOK AU - Carey, Michael F. AU - Peterson, Craig L. AU - Smale, Stephen T. TI - Transcriptional regulation in eukaryotes: concepts, strategies, and techniques SN - 9780879697624 U1 - 574.8845 PY - 2009/// CY - New York PB - Cold Spring Harbor Laboratory Press KW - Genetic Transcription KW - Regulation KW - Transcription Factors N1 - 1 A primer on transcriptional regulation in mammalian cells 2 Initial strategic issues 3 Transcription initiation site mapping 4 Functional assays for promoter analysis 5 Identification and analysis of distant control regions 6 Identifying CIS-acting DNA elements within a control region 7 Identification of DNA-binding proteins and their genes 8 Confirming the functional importance of protein-DNA interaction 9 In vivo analysis of an engogenous control region 10 Identifying and characterizing domains of transcription regulatory factors 11 DNA binding by regulatory transcription factors 12 Transcription and preinitiation complex assembly in vitro 13 Studying chromatin dynamics in vitro: chromatin assembly, remodeling, and transcription ER -