Sambrook, Joseph

The condensed protocols: from molecular cloning: a laboratory manual/ Joseph Sambrook, David W. Russell. - 1st ed. - New York: Cold Spring Harbor Laboratory Press, 2006. - v, 800p. 28 cm.

1 Plasmids and their usefulness in molecular cloning
2 Bacteriophage and its vectors
3 Working with bacteriophage M13 vectors
4 Working with high-capacity vectors
5 Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis
6 Preparation and analysis of eukaryotic genomic DNA
7 Extraction, purification, and analysis of mRNA from eukaryotic cells
8 In vitro amplification of DNA by the polymerase chain reaction
9 Preparation of radiolabeled DNA by and RNA probes
10 Working with Synthetic oligonucleotide probes
11 Preparation of cDNA libraries and gene identification
12 DNA sequencing
13 Mutagenesis
14 Screening expression libraries
15 Expression of cloned genes in escherichia coli
16 Introducing cloned genes into cultured mammalian cells
17 Analysis of gene expression in mammalian cells
18 Protein interaction technologies


Molecular Cloning
Biological Techniques
Molecular Biology

572.8 / SAM/T