TY - BOOK AU - Brown, Alfred E. TI - Benson's microbiological applications: laboratory manual in general microbiology SN - 0072823984 U1 - 576 PY - 2005/// CY - Boston PB - Tata McGraw Hill KW - Microbiology KW - Laboratory Manuals N1 - Part 1: Microscopy 1 Brightfield Microscopy 2 Darkfield Microscopy 3 Phase-Contrast Microscopy 4 Fluorescence Microscopy 5 Microscopic Measurements Part 2: Survey of Microorganisms 6 Protozoans, Algae, and Cyanobacteria 7 Ubiquity of Bacteria 8 The Fungi: Yeasts and Molds Part 3: Manipulation of Microorganisms 9 Aseptic Technique 10 Pure Culture Techniques Part 4: Staining and Observation of Microorganisms 11 Smear Preparation 12 Simple Staining 13 Negative Staining 14 Capsular Staining 15 Gram Staining 16 Spore Staining: Two Methods 17 Acid-Fast Staining: Ziehl-Neelsen 18 Motility Determination Part 5: Culture Methods 19 Culture Media Preparation 20 Preparation of Stock Cultures 21 Cultivation of Anaerobes 22 Enumeration of Bacteria: The Standard Plate Count 23 Slime Mold Culture 24 Slide Culture: Molds Part 6 25 Determination of a Bacteriophage Titer 26 Burst Size Determination: a one-step growth curve 27 Isolation of phage from files 28 Phage typing Part 7 Environmental influences and control of microbial growth 29 Temperature: effects on growth 30 Temperature: lethal effects 31 pH and microbial growth 32 Water activity and osmotic pressure 33 Ultraviolet light: lethal effects 34 Oligodynamic action 35 Evaluation of alcohol: its effectiveness as an antiseptic 36 Antimicrobic sensitivity testing: the kirby-bauer method 37 Evaluation of antiseptics: the filter paper disk method 38 Effectiveness of hand scrubbing Part 8 Identification of Unknown Bacteria 39 Morphological study of unknown bacterium 40 Cultural characteristics 41 Physiological characteristics: oxidation and fermentation tests 42 Physiological characteristics: hydrolytic reactions 43 Physiological characteristics: biochemical tests 44 Use of Bergey's manual Part 9 Miniaturized multitest systems 45 Enterobacteriaceae identification: the API 20E system 46 Enterobacteriaceae identification: the enterotube II system 47 O/F gram-negative rods identification: the oxi/ferm tube II system 48 Staphylococcus identification: the API staphident system Part 10 Diversity and environmental microbiology 49 Isolation of an antibiotic producer: the actinomyces 50 Nitrogen cycle: ammonification 51 Symbiotic nitrogen fixation: rhizobium 52 Free-living nitrogen fixation: azotobacter 53 Denitrification: paracoccus denitrificans 54 The winogradsky column 55 Purple nonsulfer plhotosynthetic bacteria 56 Sulfate reducing bacteria: desulfovibrio 57 Bacterial commensalism 58 Bacterial synergism 59 Microbial Antagonism Part 11 60 Bacterial counts of foods 61 Bacteriological examination of water: qualitative tests 62 The membrane filter method 63 Reductase test 64 Microbial spoilage of canned food 65 Microbiology of alcohol fermentation Part 12 Bacterial Genetics and Biotechnology 66 Mutant isolation by replica plating 67 Bacterial transformation 68 Polymerase chain reaction for amplifying DNA 69 Plasmid isolation Part 13 Medical microbiology and immunology 70 the staphylococci: isolation and identification 71 The streptocococci: isolation and identification 72 Gram-negative intestinal pathogens 73 Slide agglutination test: serologgical typing 74 Slide agglutination ( Latex) test: for S. aureus identification 75 Tube agglutination test: the heterophile antibody test 76 White blood cell study: the differential WBC count 77 Blood grouping 78 A synthetic epidemic ER -