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Determination of bacteria diversity and its antibiotic resistance in gradient natural thermal fields spanning across thermophilic to psychrophilic environments/

By:

Thakur, Nagendra

Material type:

TextPublication details: Gangtok, Sikkim : Sikkim University, 2022.Description: 72p.: ill. ; 29 cmSubject(s):

Contents:

Introduction 3-7 Aims and objectives 8 Review Literature 9-20 3.1. A view of different Natural thermal zones 3.2. The impacts of antibiotic resistance on human health and the environment Methodology 21-27 4.1. Sampling and site description4.2. Culture Dependent Methods 4.2.1. Isolation and characterization of bacterial isolates 4.2.2. Identification of bacterial isolates and Phylogeny 4.2.3. Antibiotic Susceptibility Testing of the bacterial isolates4.3. Culture Independent Methods 4.3.1. Elemental analysis by Inductively Coupled Plasma Mass Spectrometry 4.3.2. Metagenomic DNA extraction 4.3.3. Shotgun Metagenomic Sequencing 4.3.4. Metagenomic data analysis 4.3.5. Antibiotic Resistant Gene detection from Metagenomic sequences 4.3.6. Statistical Analysis Results 28-51 Discussion 52-60 Conclusion 61 References 62-72

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Holdings
Cover image	Item type	Current library	Home library	Collection	Shelving location	Call number	Materials specified	Vol info	URL	Copy number	Status	Notes	Date due	Barcode	Item holds	Item hold queue priority	Course reserves
	Project Report (SU)	Central Library, Sikkim University									Not For Loan			PR000004

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Introduction 3-7
Aims and objectives 8
Review Literature 9-20 3.1. A view of different Natural thermal zones 3.2. The impacts of antibiotic resistance on human health and the environment
Methodology 21-27 4.1. Sampling and site description4.2. Culture Dependent Methods 4.2.1. Isolation and characterization of bacterial isolates 4.2.2. Identification of bacterial isolates and Phylogeny 4.2.3. Antibiotic Susceptibility Testing of the bacterial isolates4.3. Culture Independent Methods 4.3.1. Elemental analysis by Inductively Coupled Plasma Mass Spectrometry 4.3.2. Metagenomic DNA extraction 4.3.3. Shotgun Metagenomic Sequencing 4.3.4. Metagenomic data analysis 4.3.5. Antibiotic Resistant Gene detection from Metagenomic sequences 4.3.6. Statistical Analysis
Results 28-51
Discussion 52-60
Conclusion 61
References 62-72

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