Microbial Biofilms: Methods and protocols/ edited by Gianfranco Donelli

Part I. Investigations on Biofilms in Health and Disease 1. Methods for Dynamic Investigations of Surface Attached in vitro Bacterial and Fungal BiofilmsClaus Sternberg, Thomas Bjarnsholt, and Mark Shirtliff 2. Aqueous Two-Phase System Technology for Patterning Bacterial Communities and Biofilms Mohammed Dwidar, Shuichi Takayama, and Robert J. Mitchell 3. Quorum Sensing in Gram-Positive Bacteria: Assay Protocols for Staphylococcal agr and enterococcal fsr SystemsAkane Shojima and Jiro Nakayama 4. Advanced Techniques for in situ Analysis of the Biofilm Matrix (Structure, Composition, Dynamics) by Means of Laser Scanning Microscopy Thomas R. Neu and John R. Lawrence 5. Multiplex Fluorescence in situ Hybridization (M-FISH) and Confocal Laser Scanning Microscopy (CLSM) to Analyse Multispecies Oral Biofilms Lamprini Karygianni, Elmar Hellwig, Ali Al-Ahmad 6. Field Emission Scanning Electron Microscopy of Biofilm-Growing Bacteria Involved in Nosocomial InfectionsClaudia Vuotto and Gianfranco Donelli 7. Experimental Approaches to Investigating the Vaginal Biofilm MicrobiomeMarc M. Baum, Manjula Gunawardana, and Paul Webster 8. Imaging Bacteria and Biofilms on Hardware and Periprosthetic Tissue in Orthopaedic InfectionsL. Nistico, L. Hall-Stoodley, and P. Stoodley 9. Animal Models to Evaluate Bacterial Biofilm DevelopmentKim Thomsen, Hannah Trostrup, and Claus Moser 10. Animal Models to Investigate Fungal Biofilm FormationJyotsna Chandra, Eric Pearlman, and Mahmoud A. Ghannoum 11. Non-Mammalian Model Systems to Investigate Fungal BiofilmsMarios Arvanitis, Beth Burgwyn Fuchs, and Eleftherios Mylonakis Part II. Investigations on anti-biofilm compounds and strategies 12. Microbiological Methods for Target-Oriented Screening of Biofilm InhibitorsLivia Leoni and Paolo Landini 13. In vitro Screening of Antifungal Compounds Able to Counteract Biofilm DevelopmentMarion Girardot and Christine Imbert 14. Biofilm Matrix-Degrading EnzymesJeffrey B. Kaplan 15. Efficacy Evaluation of Antimicrobial Drug-Releasing Polymer Matrices Iolanda Francolini, Antonella Piozzi, and Gianfranco Donelli 16. Antibiotic Polymeric Nanoparticles for Biofilm-Associated Infection TherapyWean Sin Cheow and Kunn Hadinoto 17. Pharmacokinetics and Pharmacodynamics of Antibiotics in Biofilm Infections of Pseudomonas aeruginosa in vitro and in vivo Wang Hengzhuang, Niels Hoiby, and Oana Ciofu 18. Contribution of Confocal Laser Scanning Microscopy in Deciphering Biofilm Tridimensional Structure and Reactivity Arnaud Bridier and Romain Briandet 19. Chip-Calorimetry for Evaluation of Biofilm Treatment with Biocides, Antibiotics and Biological AgentsFrida Mariana, Friederike Buchholz, and Thomas Maskow 20. Bacteriophage Attack as an Anti-Biofilm StrategySanna Sillankorva and Joana Azeredo 21. Photodynamic Therapy as a Novel Antimicrobial Strategy Against Biofilm-Based Nosocomial Infections: Study ProtocolsFrancesco Giuliani Part III. Investigations on biofilms in the environment and manufacturing plants 22. Capturing Air-Water Interface Biofilms for Microscopy and Molecular AnalysisMargaret C. Henk 23. Biofilm-Growing Bacteria Involved in the Corrosion of Concrete Wastewater Pipes: Protocols for Comparative Metagenomic AnalysesVicente Gomez-Alvarez 24. Culture-Independent Methods to Study Subaerial Biofilm Growing on Biodeteriorated Surfaces of Cultural Heritage Stone Materials and FrescoesFrancesca Cappitelli, Federica Villa, and Andrea Polo 25. Biofilms of Thermophilic Bacilli Isolated from Dairy Processing Plants and Efficacy of SanitizersSara A. Burgess, Denise Lindsay, and Steve H. Flint