Plant Immunity/ methods and protocols McDowell, John M. [ed.]

1. Studying NB-LRR Immune Receptor Localization by Agroinfiltration Transient Expression Patrick Cournoyer and S. P. Dinesh-Kumar2. Fragment Complementation and Co-Immunoprecipitation Assays for Understanding R Protein Structure and Function Peter Moffett3. Purification of Resistance Protein Complexes Using a Biotinylated Affinity (HPB) Tag Yiping Qi and Fumiaki Katagiri4. Biochemical Purification of Native Immune Protein Complexes James M. Elmore and Gitta Coaker5. Chromatin Immunoprecipitation (ChIP) to Identify Global Targets of WRKY Transcription Factor Family Members Involved in Plant Immunity Mario Roccaro and Imre E. Somssich6. Dose Response to and Systemic Movement of Dexamethasone in the GVG Inducible Transgene System in Arabidopsis Xueqing Geng and David Mackey7. Quantifying Alternatively Spliced mRNA via Capillary Electrophoresis Xue-Cheng Zhang and Walter Gassmann8. Constructing Haustorium-Specific cDNA Libraries from Rust Fungi Ann-Maree Catanzariti, Rohit Mago, Jeff Ellis, and Peter Dodds9. Microaspiration of Esophageal Gland Cells and cDNA Library Construction for Identifying Parasitism Genes of Plant-Parasitic Nematodes Richard S. Hussey, Guozhong Huang, and Rex Allen10. Construction of Pseudomonas syringae pv. tomato DC3000 Mutant and Polymutant Strains Brian H. Kvitko and Alan Collmer11. A Straightforward Protocol for Electro-Transformation of Phytophthora capsici Zoospores Edgar Huitema, Matthew Smoker, and Sophien Kamoun12. Propagation, Storage, and Assays with Hyaloperonospora arabidopsidis, a Model Oomycete Pathogen of Arabidopsis John M. McDowell, Troy Hoff, Ryan G. Anderson, and Daniel Deegan13. Assaying Effector Function In planta Using Double-Barreled Particle Bombardment Shiv D. Kale and Brett M. Tyler14. Assays for Effector-Mediated Suppression of Programmed Cell Death in Yeast Yuanchao Wang and Qian Huang15. Purification of Effector-Target Protein Complexes via Transient Expression in N. benthamiana Joe Win, Sophien Kamoun, and Alexandra M.E. Jones16. Imaging Fluorescently-Tagged Phytophthora Effector Proteins Inside Infected Plant Tissue Petra C. Boevink, Paul R.J. Birch, and Stephen C. Whisson17. Immunolocalization of Pathogen Effectors Eric Kemen, Kurt Mendgen, and Ralf T. Voegele18. Laser Capture Microdissection of Nematode Feeding Cells Nagabhushana Ithal and Melissa G. Mitchum19. Laser Microdissection of Plant-Fungus Interaction Sites and Isolation of RNA for Downstream Expression Profiling Divya Chandran, Noriko Inada, and Mary C. Wildermuth20. Global Expression Profiling of RNA from Laser Microdissected Cells at Fungal-Plant Interaction Sites Divya Chandran, Greg Hather, and Mary C. Wildermuth21. Visualizing Cellular Dynamics in Plant-Microbe Interactions Using Fluorescent-Tagged Proteins William Underwood, Serry Koh, and Shauna C. Somerville