Recent trends in horticultural biotechnology/ edited by R Keshav Chandran ...[et al]

Foreword - Prof. V.L Chopra
XI
Contents
Acknoxoledgement dH
Preface iv
SESSION II: Molecular Markers and their Exploitation
77. Molecular Diversity among South East Asian Coconut
{Cocos nucifera L.) Germplasm Accessions Based on ISSR Markers
R. Manimekalai, P. Nagarajan, M. Bharathi, Anitha Kanin
P.M.Kumaran and V.A.Parthasarathy 479
78. Assessment of Genetic Variability in Black Pepper
{Piper nigrum L.) Varieties Through RAPD and AFLP Analyses
P.A. Nazeem, R. Keshavacliandran, T.D. Babu, C.R.Achuthan
D. Girija and K.V.Peter 485
79. Assessment of Genetic Diversity and Relationships Among
Some Grape Varieties using ISSR PGR Based Markers
Manjusha Dhane, S.A.Tamhankar, S.G. Patil
G.S. Karibasappa and V.S. Rao 491
80. AFLP Polymorphism and Pythium Rresponse in Zingiber species
Kavitha, P.G., Pratibha Nair, Aswati Nair, R, Jayachandran
B.K, Sabii, M. and George Thomas 497
81. Marker Assisted Selection for Purple Blotch Disease
Resistance in Onion {Alliiim cepa L.).
Ganesh C. T. and Veeregowda, R. 505
82. Genetic Diversity in Some Wild and Cultivated Germplasms
in VIGNA by RAPD Marker
B. Roychoudhury,Sxvagata Ghosh, N. Mandal and T. Dasgupta 515
83. DNA Fingerprinting in Tapioca (Manihot esculenta crantz.)
Santha V. Pillai and S. Sundaresan 525
84. RAPD Analysis of Monoclonal and Polyclonal Seedlings of
Hevea brasiliensis
P.Sobhana, Molly Thomas and James Jacob 531
XII
85. Genetic Divergence Analysis of Nendran (AAB) licotypes of
Banana (Musn spp.) using RAPD Marker and Morphological Traits
C Rnjmmnicknm and K. Rajniohnti ' 537
86. RAPD Analysis of Banana (Musa AAB Plantain subgroup) Clones
Simi, S., K. Rnjmolmn and K.B. Soni 545
87. Molecular Characterization of Psidiiim spp. using RAPD Markers
Ansliii SJmrmn, K.S. Boom, R.S. Singlirot and S.K. Sclimwat 553
88. Molecular Characterization of Ber (ZizipJnis nmiihtinna Lamk)
Germplasm
S. Raja, S.K. Sehrnwat, K.S. Boom and D.S. Daliiija 559
89. Application of RAPD Technique to Study the Genetic Relationship
of Orchids
Syamali Chakrabarti 5^5
90. Detection of Genetic Variability in Ashgourd
{Benincasa hispida (Thunb.) Cogn.) Using RAPD Markers
/. Resmi, I. Sreelatliakntnanj and K. Rajniohan 571
91. Application of RAPD in Characterization of Germplasm
Including a Novel Segmented Leaf Genotype in Bottle Gourd
Hari Har Ram and Kavita Shanna 579
92. Detection of Genetic Variability in Drumstick (Moringa oleifem lam.)
• using RAPD Markers
D. S. Resmi, V. A. Celine, L. Rajamony, K. B. Sony 587
93. RAPD Analysis of Centella asiatica Ecotypes
G.R. Pradeep Krishnan, C.N. Vishnu Pmsad, K. Rajrnohan
and K.B. Soni 593
94. Fingerprinting and Genetic Diversity of Purple Blotch Disease
Resistant and Susceptible Genotypes of Onion (Allium cepa L.)
using RAPD Markers.
Ganesh C. T. and Veeregowda, R. 599
95. Screening of PGR Primers for Oil Palm (Elaeis guineensis Jacq.)
Shell Thickness Marker
P.K.Mandal and R.S.N.Pillai 613
96. Development of Protocols for DNA Extraction and Amplification
in Onion {Allium cepa L.) for RAPD Analysis
Ganesh, C.T., Veere Gowda, R., Narayanaswamy P.
and Ramanjini Gowda, P. H. 619
97. Standardizcition of Gciieration of Random Amplified Polymorphic
DNA's in Chrysanthemum
S.K. Selirnwat, K.S. Boom, Rmijit Kuomr, D.S. Daliiyn
(Did R.K. Goynl ^27
98. Genetic Fingerprinting of Piper nigruin L. and Piper longuin L.
Cultivars using RAPD Markers
R.Keshavnchandrmi, P.A.Nnzeeiu and I.LKnrilialoo 635
99. Genetic Basis for Sex Determination in Date Palm
{Phoenix dnctylifera L.)
Rnjendrmi, P mid IhrnJiiiii Snker AJ-Mssnlleiii 641
100. Study of Isozyme Polymorphism in Musa Cultivars of Kerala
Retm Menoii, KM. Sunny, Nishn Jose, PA. Nnzeeni
R. Keslinvachandran and Shakunthnla Nnir 649
SESSION III: Molecular Cloning of Genes
101. Isolation, Cloning and Characterization of Genes Encoding
Fungal Endochitinase
Sniprasad, G.V.S., MytJiili, J.B., Lnlithn Annnd, Snneetha, C.
and Rashmi, H.J. 653
102. Molecular Cloning of a cDNA Fragment Encoding
Hydroxy Methyl Clutaryl CoA Reductase in Piper colubrimim
D. Girija, P.S. Beena, P.A. Nazeem and M.G. Puroshothama 663
103. Cloning of Endochitinase Gene from Native Isolates
of Trichoderma Spp,
Aishwarya R. Anigol, Siimangala Bhat and M.s. Kumvinashetti 671
104. Cloning and Expression of Prophenoloxidase (PPO) gene
and its Activating Enzyme from Heliothis arniigera
Bindiya Sachdev, Neerna Agrawal, Tarannuni Ahmad
S.Sivakumar and R. K. Bhatnagar. 683
105. Cloning of Coat Protein Gene of Kursupara Isolate of Cardamom
mosaic Virus for Developing Transgenic virus-resistant cardamom
S.Backiyarani, C.Manohari, T.Jebasingh, Thomas Jacob and R.Usha 693
106. Cloning of crylAb Gene from a New Indigenous Isolate
of Bacillus thuringiensis
Devendra Jain, Sana S.Dev, P. Indra And Selvi and
V. Udayastiriyan 699
XIII
XIV
SESSION IV: Genetic Modification for Crop Improvement
107. Studies on The Influence of Cytokinin Independent Gene
(CKIl) on Explant Differentiation in Tomato a\ Arka Vikas
Through Agrohacteriinn Mediated Transformation
MytJiili, ].B, Narasimlin Miirtlnj, D.K and Lalitha Auand 703
108. Agrobacterium-Medidded Transformation using Green
Fluorescence Protein (GPP) in Papaya (Cnricn papaya L.)
N.N. Renukdas and S.K.Rawal 711
109. Factors Affecting Agrobacterium tiimefaciens Mediated
Transformation in Ginger (Zingiber ofpcinale Rose.)
Siima, B. and Keshavachandran, R. 721
110. Tuber Specific Overproduction of Cytokinins Results in Enhanced
Tuber Number in Transgenic Potato
Nisha K. K. and Punisliothama M. G. 727
111. Agrobacterium Mediated Genetic Transformation in Dendrobium
R. Szvarnapiria, K. RajmoJian 731
112. Development of Transgenic Cassava : Optimization of Protocol
for Agrobacterium Mediated Gene Transfer
Sheela, M.N. and Nigel J. Taylor 739
113. In Vitro Regeneration and Transformation of Coleus forskohlii
With Glucanase-Chitinase Gene
Miss Khin My at Lwin, Ramanjini Gowda P.H, T.K.S. Goxoda
Nageslm N, Sreenevasa.S. Manjunatha. T. V.U Patil
Vasundhara. M, and Chandrashaker. S.C 74:7
114. In Vitro Micro Propagation and Transformation of Contoloupe
(Cucumis melo L.) with GUS Gene Through Agrobacterium
Mediated Transformation
Nagesha N., Devaiah B.N., Ramesh B.N, and Ramanjini Gowda P.H. 753
115. Transformation Studies in Chilli {Capsicum annuiim L.)
Jayapadma P.N., Sumangala Bhat and M.S. Kuruvinasbetti 761
116. Agrobacterium Mediated Genetic Transformation in Bell Pepper
{Capsicum annuum L.)
A.G. Fatima, D. Girija, C.S. Meera, P.A. Nazeem and
Lissamma Joseph . 765
117. Induction of Hairy Roots in Holostemma ada-kodien with
Agrobacterium rhizogenes
S.H. Karmarkar and R. Keshavachandran 769
SESSION V: Plant Microbe Interactions
118. Novel Diastereomeric Antifungal Peptides against PJn/tophfhora capsici
the Causative Agent of Quick Wilt Disease of Black Pepper
Mnnoj, K. /., Suresh Kumar, U., Santliosh Kumar, K.
Anandaraj, M. and George Thomas 773
119. Induction of Systemic Resistance Against Die-back and
Fruit Rot of Chillies by Pseudomonas fluorescens
B. Meena and T. Marimuthu yg^
120. Potential of Facultative Methylotrophs in Increasing
the Yield of Tomato Crop
G.Thangamani and SP.Siindaram 739
121. Efficacy of Rhizosphere Microflora from Black Pepper Nursery
against Phytophthora Rot Pathogen
Reshmy Vija\/aragJiai)an and KosJiy Abraham 797
122. Studies on The Impact of Bio-Fertilizers with Levels of NPK
on Onion {Allium cepa L.) cv. Bellary Red
Mahanthesh, B., Venkatesha, }., Radhakrishna, D., Umesha, K.
and Veere Gowda, R. gQ5
SESSION VI: Environmental and Industrial Biotechnology
123. Ethylene Mediated Modulation of Glucanase Activity in Ginger
{Zingiber officinale Rose.)
P. Deepfhy Antony, Thomas, G. and M.G, Purushothama 813
124. Screening of Mesorhizobiiim ciceri Isolates for
Development of Biofertilizer Strains for Chickpea Production
Kanika, R.K. Manila and L.R, Kashyap 817
125. Effect of Microbial Antagonists on the Management of Bacterial
Wilt in Tomato
Sally K. Mathew, Manimala, R., Surendra Gopal, K. and
Nirmala Devi, S. g23
126. Biochemical Basis of Shoot and Fruit Borer {Leucinodes orbonalis G.)
Resistance in Brinjal {Solarium melongena L.)
M.Prabhu, S.Natarajan and Pugalenthi 829
127. Vesicular Arbuscular Mycorrhizae (YAM) Enhances the
Drought Stress Tolerance of Sesbania sesban
Jos T Puthur ggy

128. Effect of Soil Solarization and Biocontrol Agents in Growth
Enhancement of Black Pepper Cuttings
Reslimy Vijayaraghavan, Kosliy Abraham and Dccpa James 849
129. Influence of Beneficial Microbes on Damping off Disease
and Seedling Vigour in Solanaceous Vegetables
Sally K. Matlow, Sadankumar, P.G., Nirmala Dei'i, S.
ami Krishnan, S.
130. Role of Pseudomonas fluorescens in the Integrated Management
of Rhizome Rot Disease of Banana.
Usha.N.K and Anita Clierian K
131. Potential of Rhizobacteria in the Management of Soil Borne
Pathogen Rhizoctonia solani Kuhn.
Rcliiimatli Niza, T.j., Saif Ncesa, T.K., Sally K. Mathew
Koshy Abraham and Siirendra Gopal, K. 865
132. Efficacy of Selected Epiphytic Microflora from Pod Surface
against Phytophthora Pod Rot of Cocoa
R. Bhavani and Koshy Abraham
133. Use of Metachromasia to Study the Chemical Nature of LPS from
Specific and Non-Specific Strain
Ganesh K.S and Uma S. Podder
134. Evaluation of Fungal Antagonists against Leaf Spot Pathogens
of Ivy Gourd {Coccinia grandis (L.) Voigt)
Deepa Davis, S. Beena, Sally K. Mafhew and Koshy Abraham 883
135. Mechanism of Antagonism of Selected Epiphytic Antagonists
against Phytophthora palmivora: The Incitant of Pod Rot of Cocoa
R. Bhavani and Koshy Abraham
136. Efficacy of Antagonists Against Bacterial and Fungal Pathogens
of Kacholam {Kaempferia galanga L.)
K Priya, Sheela Paul T and Beena S
137. Participatory Assessment of Native Trichoderma Isolates in Newly
Planted Pepper Gardens of Kerala
Rehumath Niza, T.j., Koshy Abraham, Nybe, E.V.
and Prince, E.J.
138. Induction of Mutation in Trichoderma virens using UV-Irradiation
Top G. Tharaniyil and P.K. Mukherjee

139. Mol6culcir CharactGrization of An Endophytic NitrogGn Fixing
Bacterium Associated with Black Pepper
D. Giiijn, A/1. B. Bindu, P.A. Nazeetii, G. Sunil KrisHna and
Jasiri Inyasiiuliaji
140. Characterization of Plant Growth Promoting Bacillus sp.
from Chickpea Rhizosphere
Sivarnmainh, N, Sindhu, S.S mid Dadnnuai K.R. 917
141. Arbuscular Mycorrhizal Fungal Association in Different
Species of Sesame
Svenip John, AAmioj Sebastian, D.Alexander, Shyani S.Kump
and K. Rajmo Jian ^25
142. Biochemical Response of Tomato Calli of Resistant Genotypes
to the Crude Toxins of Foliar Pathogens
Relnimatli Niza, T.j. and Narayanasaniy, P. 929
143. Selective Retention of Cadmium and Lead in Tomato
(Lycopersicon escnlentiun Mill.)
Vanisri, K., Sam T. KiinimtJiottical and AAarykutty, K.C. 935
144. Mitochondria Isolation, SDH Assay and Protein ProfUing in
Potato [Solanuni tiiherosuni L.) Tubers for Improving Shelf Life
Pradeep Kumar Shiikla, P. Misra, Gurdeep Bains, S.K. Gum
Alok Shukla and R.C. Pant 94I
145. Lipid Isolation and Characterization in
Mung Bean (Vigna radiata L.) roots
Pragati Mishra, P. K. Shukla, S.K. Gum, Alok Shukla and R.C. Pant 947
146. Biochemical Investigations of Enriched Coir Pith Compost Materials
Sushama, P.K. and Hari, T.K. 957
SESSION VII: Bioinformatics and Biosafety Issues of
Transgenic Crops
147. Homology Modeling of CrylAc and its Comparison with
Receptor Binding Region of CrylAa
Arunkumar. K. Ramasamy and V. Udayasuriyan 963
148. Bioinformatics - a KAU Perspective
Keshavachnndran, R, Ajitha T.K, Lavina C.V, Deepthy P.S
Nazeem P.A and Girija D 957