Molecular biology and biotechnology: a guide for students/ Helen Kreuzer

1 An Overview of Biotechnology 3
2 Cell Properties and Processes 45
3 Genes, Genetics, and Geneticists 73
4 An Overview of Molecular Biology 97
5 Recombinant DNA Technology 137
PART II
Classroom Activities 169
A. DIVA Structure and Function 171
6 Student Activity: Constructing a Paper DNA
Model 172
7 DNA Replication 174
8 Student Activity: From Genes to Proteins 176
Student Activity: Making Antisense: Regulating
Gene Expression with RNA 179
9 Student Activity: Sizes of the Escherichia coli and
Human Genomes 182
Student Activity: Extraction of Bacterial DNA 18510
B. Manipulation and Analysis
of DIVA 187
11 Student Activity: DNA Scissors 188

Student Activity: DNA Goes to the Races 190
Student Activity: Restriction Analysis of
Lambda DNA 194
Student Activity: Recombinant Paper Plasmids 199
Student Activity: Restriction Analysis
Challenge 202
Student Activity: Detection of Specific DNA
Sequences: Part I. Fishing for DNA '205
Student Activity: Detection of Specific DNA
Sequences: Part II. Combining Restriction and
Hybridization Analysis 207
Student Activity: Detection of Specific DNA
Sequences: Part III. Southern Hybridization 209
Student Activity: Paper PCR 210
Student Activity: DNA Sequencing: The
Terminators 214
Reading: Chain Terminators as Antiviral
Drugs 218
C. Transfer of Genetic Information 221
19 Student Activity: Transformation of Escherichia
coli 222
20 Student Activity: Conjugative Transfer of Antibiotic
Resistance in Escherichia coli 225
Reading: Gene Transfer and the Spread of
Antibiotic Resistance 228
21 Suede}il Activity: Transduction of an Antibiotic
Resistance Gene 234
22 Student Activity: Agrobacterium tumefaciens:
Nature's Plant Genetic Engineer 238
D. Molecular Biology and Genetics 24l
23 Student Activity: An Adventure in Dog Hair,
Part 1 242
24 Student Activity: An Adventure in Dog Hair. Part II:
Yellow Labs 246
25 Student Activity: Human Molecular Genetics 250
Reading: Molecular Genetics of Cancer 258
26 Student Activity: Genetics in Action 264
£. Genomics 271
27 Student Activity: Comparing Genomes 272
Exercise 1: STRs Can Cause an RFLP 278
Exercise 2: PCR Can Reveal Differences at
Microsatellite Loci 279
Reading: Mitochondria] DNA 281
28 Student Activity: Forensic DNA Typing 283
Exercise 1. A Mix-Up at the Hospital 285
Exercise 2. A Paternity Case 285
Exercise 3. The Case of the Bloody Knife 286
Reading: DNA Forensics: An Archaeological
Case 289
29 Student Activity: Mapping a Disease Gene 290
Reading: The Human Genome Project: Its Science,
Applications, and Issues 299
30 Student Activity: Microarray Analysis of Genome
Expression 303
Reading: Personal Genomics 306
F. Bioinformatics and Evolutionary
Analysis of Proteins 309
31 Testing for Amylase Activity 310
Student Activity: Electrophoresis of Amylase
Samples 315
Student Activity: Constructing an Amylase
Evolutionary Tree 318
Student Activity: Exploring Bioinformatics 322
VII
PART III
Societal Issues
329
40 Stiicli'nt Activity: Anal\ ziiij> Mthit al Dilcminas in
CiL-ne Therapy i
Introduction to Societal Issues 331
35 Student Activity: Facing the Forces; Science,
Technology, and Society 332
Reading: The Nature of Science 338
36 Student Activity: Analyzing the Issues of
Biotechnology: Cloning 341
Reading: Are Bioengineered Foods Safe? 352
37 Student Activity: The Risks of Technology:
Perception, Reality, or Bcnh? 356
Reading: Biotechnology Regulation in the United
States 368
38 Student Activity: Making Decisions When There Is
No Right Answer 372
39 Student Activity: Analyzing Ethical Dilemmas in
Genetic Testing 377
Reading: Science, Law, and Politics 381
PART IV
Appendixes
A Worksheets 390
389
B Laboratory Biosafety •i53
C Aseptic 'Technique 155
Glossary 457