Molecular markers and plant biotechnology/ Rukam S. Tomar

1. Milestones in DNA history and biotechnology. 2. Equipments required in a molecular marker laboratory. 3. Isolation, purification and quantification of genomic DNA from plants. 4. Isolation, purification and quantification of RNA from plants. 5. Electrophoresis. 6. Molecular weight markers for gel electrophoresis. 7. Polycrylamide Gel Electrophoresis PAGE. 8. Gel electrophoresis of protein. 9. Isoenzyme. 10. Extraction of DNA fragments from Agarose Gel. 11. Why molecular markers? 12. Restriction enzyme digestion of DNA and its Agarose Gel Electrophoresis. 13. Thermocyclers PCR Machines. 14. Modifications of basic PCR technique. 15. Random amplified polymorphic DNA RAPD. 16. Restriction Fragment Length Polymorphism RFLP. 17. Amplified Fragment Length Polymorphism AFLP. 18. Simple sequence repeats Microsatellite. 19. Variable number of Tandem repeats Minisatellite. 20. Inter Simple Sequence Repeat ISSR. 21. Sequence Characterized Amplified Region SCAR. 22. Cleaved Amplified Polymorphic Sequence CAPS. 23. Single-Strand Conformation Polymorphism SSCP. 24. Retrotransposon-based markers S-SAP, IRAP, REMAP, RBIP, RGAS, SNP. 25. Silver staining. 26. Basics of marker assisted selection in crop plants. 27. Mapping populations. 28. QTL mapping. 29. Southern blotting. 30. Western blotting. 31. Northern blotting. 32. Eastern blotting. 33. DNA sequencing and designing of primers. 34. Gene transfer to plant. 35. Fluorescent in Situ Hybridization FISH. 36. Genotypic Barcoding. 37. mtDNA. 38. MicroRNA. 39. Graphical approach to calculate molecular weights of DNA/protein fragments. 40. Calculation of similarity index values and construction of Dendrogram using NTSYSpc 2.0.