Laboratory and Field Manual for Plant Nematology/ (Record no. 176476)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 00393nam a2200133Ia 4500 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9788190757706 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CUS |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 571.999 |
| Item number | RED/L |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Reddy, P. Parvatha |
| 245 #0 - TITLE STATEMENT | |
| Title | Laboratory and Field Manual for Plant Nematology/ |
| Statement of responsibility, etc. | P. Parvatha Reddy |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | New Delhi: |
| Name of publisher, distributor, etc. | Studium Press, |
| Date of publication, distribution, etc. | 2009. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | xiv, 260p. |
| 505 ## - FORMATTED CONTENTS NOTE | |
| Formatted contents note | <br/>1.NEMATOLOGYEQUIPMENT 1<br/>1.1. LaboratoryEquipment 1<br/>1.1.1. Furniture and Fittings 1<br/>1.1.2. Microscopesand Equipment 3<br/>1.2. Field Equipment 5<br/>1.3. Library 5<br/>1.3.1. Books 5<br/>1.3.2. Journals 7<br/>1.3.3. Internet Websites 8<br/>2.MICROSCOPE 9<br/>2.1. Stereoscopic Microscope 9<br/>2.1.1. Illumination ofDissecting Microscope 9<br/>2.1.2. Use 10<br/>2.2. Compound Microscope 11<br/>2.2.1. Components 11<br/>2.2.2. Use 13<br/>2.2.3. Alignment and Care 13<br/>2.2.4. Drawing and MeasuringofNematodes 14<br/>2.3. scanning electron Microscope(SEM) 18<br/>2.3.1. AdvantagesofSEM 18<br/>2.3.2. Use 19<br/>3.SAMPLING NEMATODEPOPULATIONS 21<br/>3.1. SamplingProcedures' 21<br/>3.1.1. Detection and Survey 21<br/>3.1.2. Diagnosis or Detection 21<br/>3.1.3. Advisory 22<br/>3.1.4. Research 22<br/>3.2. Collection of samples 22<br/>3.2.1. sampling equipment 22<br/>3.2.2. Time of sampling 23<br/>3.2.3. Sample Size 23<br/>3.2.4. SamplingPatterns 23<br/>3.3. Care and Conditioning ofSoil and plant tissue Samples 25<br/>3.3.1. Handling of samples during Collection and Transit 25<br/>3.3.2. Storage 25<br/>3.3.3. Storage Temperature and Moisture 25<br/>3.3.4. Storage Time 2$<br/>3.4. Fact Sheet 26<br/>3.4.1. Nematode Survey 2uid Collection 26w<br/>^ COTltCFltS \f.<br/>4.EXTRACTION OF NEMATODESFROMSOIL 29<br/>4.1. Cotton Wool Filter Technique 30<br/>4.2. Decanting and Sieving Technique 31<br/>4.3. Simple Extraction Method for Large Nematodes like 33<br/>Xiphinema and Longidorus<br/>4.4. Two-Flask Technique<br/>4.5. Elutriation Techniques<br/>4.5.1. Gostenbrink Elutriation System<br/>4.5.2. Seinhorst Elutriation System<br/>4.6. Centrifugal Flotation Technique<br/>4.7. Estimating Nematodesin Aqueous Suspensions<br/>4.8. British Standard Sieves<br/>5.EXTRACTION OFCYSTNEMATODESFROMSOIL<br/>5.1. A Simple Extraction Method<br/>5.2. Acetone or Acetone: Carbon Tetrachloride Method<br/>5.3. Paper Strip Method<br/>5.4. Kirchner's Funnel Method<br/>5.5. Modified Fenwick Can Method<br/>5.6. DPI Cyst Separation Technique<br/>5.7. Gostenbrink's Flotation Apparatus<br/>5.8. Steinhorst's Extraction Apparatus for Cystsfrom Moist Soil<br/>5.9. Reinmuth's Centrifuging Method<br/>5.10. Centrifugal Flotation Technique<br/>5.11. Separation ofCystsfrom Admixtures<br/>5.12. Estimation ofEgg contentofCysts<br/>5.13. HatchingTests on Heterodera/Globodera Cysts<br/>5.14. Perspex Slides for Mounting Cyst Nematodes<br/>6.EXTRACTIONOFNEMATODESFROMPLANTMATERIAL<br/>6.1. DissectingPlant Materialien Water<br/>6.2. Modified Baermann FunnelTechnique<br/>6.3. MacerationyFiltration Technique<br/>6.4. RootIncubation Technique<br/>6.5. Mistifier Technique<br/>6.6. A Centrifugal Flotation Method for Recovering Nematodes'<br/>and Eggs from Plant Tissue<br/>6.7. Modified Centrifugal Flotation Method<br/>6.8. Maceration-sedimentation Technique for Recovery of<br/>Rhadinaphelenchuscocophilusfrom CoconutPalm Tissue<br/>6.9. Extraction ofSedentary Nematodes and Eggs<br/>6.10.Mechanical Maceration Technique<br/>6.11.Enzjrme and Chemical Maceration Technique<br/>6.12.Fact Sheet<br/>6.12.1. ProcessingofRootand Soil SamplesLaboratory&Field ManualforPlantNematology xi<br/>7.EXTRACTION OF EGGS 73<br/>7.1. From Roots 73<br/>7.1.1. With Few Exposed Eggs 73<br/>7.1.2. With ManyExposed Eggs ' 73<br/>7.2. From Soil 75<br/>7.2.1. Root-knot Nematode Eggs 75<br/>7.2.2. Free-living Nematode Eggs 76<br/>7.3. Hatching £ind Collection of Meloidogyne spp.Juveniles 77<br/>7.4. Procedure for Collection,Extraction and Calibration of 77<br/>Root-knot Nematode Eggsfor Inoculum<br/>8.HANDLING NEMATODES 79<br/>8.1. Pickingand TransferringIndividualNematodes 79<br/>8.2. Preparing SharplyPointed Metal Needles 79<br/>8.3. Anaesthetization 80<br/>8.4. Killingand Fixing 80<br/>8.4.1. Fixatives 82<br/>8.4.2. Properties ofMostImportant Fixatives 83<br/>8.5. Preparation of nematode Moments 84<br/>8.5.1. Mounting Nematodes in Water 84<br/>8.5.2. Temporary Mounts 85<br/>8.5.3. Semi-permanent mounts 85<br/>8.5.4. Permanent Mounts 86<br/>8.6. Glycerol Mounts for Free-living Nematodes 89<br/>8.7. Aluminium Double Cover Slip Slide 90<br/>8.8. Fixation and Preservation of plant Material 90<br/>8.9. Preparation ofPosterior Cuticular Pattern of root-knot 91<br/>Nematodes<br/>8.10. Preparation ofVulval Cone of cyst Nematodes 93<br/>8.11. Preparation of Face View ofNematodes 94<br/>8.12. Preparation ofDissected Specimens 95<br/>8.12.1. Dissecting Stylets ofMeloidogyne Females 95<br/>8.12.2. Dissecting Stylets ofMe/oido^yne Males and 97<br/>Second-stage juveniles<br/>8.12.3. Dissecting Stylets of other Nematode Genera 97<br/>8.12.4. Dissecting Spicules 97<br/>8.13. Prep£iration of issues and Cells 98<br/>8.13.1. Dissection of sperm from Seminal Vesicle ofMale 98<br/>8.13.2. Dissection of Sperm from Inseminated Females 98<br/>9.STAINING 101<br/>9.1. Staining ofRoots 101<br/>9.1.1. Staining by Fleming's Reagent 101<br/>9.-1.2. Staining with Picric acid and Aniline Blue for 102<br/>Hardwood Roots<br/>9.1.3. Staining with Bromophenol or Bromothymol Blue 102i<br/>xii Contents |<br/>5;<br/>9.1.4. Staining in Aceto-osmium 103 |<br/>9.1.5. Staining in Lugol's Solution ' 103<br/>9.1.6. Sodium Hypochlorite(NaOCl)-Acid Fuchsin Method|103<br/>9.1.7. Differential StainingofEndo-parasitic nematodes 104<br/>9.2. Staining Shoots 104<br/>9.2.1. Staining with Cotton Blue or Acid Fuchsine in Lacto- 104<br/>phenol<br/>9.2.2. Staining with Acid Fuchsin-Glycerol-Lactic Acid 105<br/>9.2.3. Staining with Fleming's Reagent 105<br/>9.3. Stains for Distinguishing Live from Dead Nematodes 106<br/>9.3.1. Staining with New BlueR 106<br/>9.3.2. Staining with Chrysoidin 106<br/>9.3.3. Staining with PhloxineB 106<br/>9.3.4. StguningwithEosin-Y 106<br/>9.3.5. Staining with Acridine Orange 106<br/>9.4. Specialized Staining 107<br/>9.4.1. Staining with Acetic Orcein 107<br/>9.4.2. Staining with Gold Chloride 107<br/>9.4.3. Staining with Silver Nitrate 107<br/>9.4.4. Silver Staining for Faint Surface Structures of 107<br/>Nematodes<br/>9.4.5. Staining of eggs and Egg Sacs with Phloxine B 108<br/>10.MICROTOMESECTIONING 109<br/>10.1. Killing and Fixation 110<br/>10.2. Dehydration HI<br/>10.2.1. Dehydration by Non-solventsofParaffin 111<br/>10.2.2. Dehydration by Solvents ofParaffin 111<br/>10.3. Infiltration and Embedding 112<br/>10.4. Microtome Sectioning 112<br/>10.5. Staining 113<br/>11.CULTURINGNEMATODES 117<br/>11.1. SterilizingNematodes 117<br/>11.2. SterilizingEggMasses 119<br/>11.3. SterilizingSeeds 120<br/>11.4. Mass Culturing of mushroom Nematode{Ditylenchus 121<br/>mycetophagous)<br/>11.5. Mass Culturing oiAphelenchoidesritzemabosi 122<br/>11.6. Mass Culturing ofDitylenchus destructor 122<br/>11.7. Mass Culturing ofGlobodera rostochiensis and G.pallida 123<br/>11.8. Mass Culturing of Meloidogyne spp.on Excised Tomato Roots 124<br/>11.9. Mass Multiplication of root-knot Nematodes on Tomato Plants 131<br/>11.10.CultxiringofRadopholussimilis andPratylenchusspp.on Carrot 132<br/>Discs<br/>11.11.A Culturing Method for Nematodes on an Aerated Nutrient 137<br/>AgarMixtureLaboratory&Field ManualforPlantNematology xiii<br/>12.SCREENING OF GERMPLASM 141<br/>12.1. Screening ofBanana Germplasm against Migratory<br/>endoparasitic Nematodes(Pratylenchus coffee and<br/>Radopholussimilis)under Greenhouse Conditions 141<br/>12.1.1. Equipment and Materials 141<br/>12.1.2. Preparation of Plant Material 142<br/>12.1.3. Extraction and Inoculation with Nematodes 142<br/>12.1.4. Data Collection 143<br/>12.2. Screening ofBanana Germplasm against root-knot 147<br/>Nematodes{Meloidogyne spp.)under Greenhouse<br/>Conditions<br/>12.2.1. Equipment and Materials 147<br/>12.2.2. Preparation of plans Material 147<br/>12.2.3. Extraction and Inoculation with Nematodes 148<br/>12.2.4. Data Collection 148<br/>12.3. Screening ofBanana Germplasm againstNematodes 151<br/>imderField Conditions<br/>12.3.1. Equipment and Materials 151<br/>12.3.2. Preparation of Plant Material 152<br/>12.3.3. Site Selection and Preparation 152<br/>12.3.4. Transfer to Field 152<br/>12.3.5. Data Collection 153<br/>13.MASSPRODUCTIONOFBIOLOGICALCONTROLAGENTS 157<br/>13.1. Bacteria 157<br/>13.1.1. Pasteuriapenetrans 157<br/>13.1.2. Pseudomonasfluorescens 160<br/>13.1.3. Rhizobacteria(Psewdo/nonasspp.,BaciZZus spp., 161<br/>Serratia spp.,etc.)<br/>13.1.4. EndophyticBacteria 162<br/>13.1.5. Streptomyces avermitilis(Avermectins) 164<br/>13.2. Fungi 168<br/>13.2.1. Nematode Trapping or Predatory Fungi 168<br/>13.2.2. Trichoderma viride and T.harzianum 172<br/>13.2.3. Paecilomyceslilacinus 177<br/>13.2.4. Arbuscular mycorrhizal fungi(AMF) 178<br/>13.2.5. EnrichmentofFYMwithBioagentsCTrZcAocZerma 182<br/>harzianum^Paecilomyceslilacinus)<br/>13.2.6. Seed Biopriming/Solid MatrixPriming 184<br/>13.2.7. Formulation ofAlginated Pellets ofBiocontrol Agents 184<br/>13.2.8. Preservation ofBiocontrol Agents 184<br/>13.2.9. Production ofBioagent Colonized Seedlings 187<br/>13.3. Entomopathogenic Nematodes(EPN) 187<br/>13.3.1.In vivo Production 188<br/>13.3.2. In vitro Production 191<br/>13.3.3. Formulation 193XIV<br/>14.roENTIFICATIONOFCOMMON GENERA OF<br/>PLANTPARASITICANDENTOMOPATHOGENIC<br/>NEMATODES<br/>14.1. Plant Parasitic Nematodes<br/>14.1.1. Tylenchorhynchus spp.(StuntNematodes)<br/>14.1.2. Ditylenchus spp.(Stem and Bulb Nematodes)<br/>14.1.3. Belonolaimus spp.(Sting Nematodes)<br/>14.1.4. Hoplolaimusspp.(Lance Nematodes)<br/>14.1.5. Helicotylenchus spp.(Spiral Nematodes)<br/>14.1.6. Rotylenchulus spp.(Reniform Nematodes)<br/>14.1.7. Pratylenchus spp.(Lesion Nematodes)<br/>14.1.8. Hirschmanniella spp.(Rice-rootNematodes)<br/>14.1.9. Ra4opholus spp.(Burrowing Nematodes)<br/>14.1.10. Meloidogyne spp.(Root-knot Nematodes)<br/>14.1.11. Heterodera and Globodera spp.(CystNematodes)<br/>14.1.12. Criconemoidesspp.(Ring Nematodes)<br/>14.1.13. HemicriconeTnoidBs spp.(Sheathoid Nematodes)<br/>14.1.14. Hemicycliophora spp.(Sheath Nematodes)<br/>14.1.15. Tylenchulus spp.(Citrus RootNematodes)<br/>14.1.16. Paratylenchus spp.(Pin Nematodes)<br/>14.1.17. Anguina spp.(Seed Gall Nematodes)<br/>14.1.18. Aphelenchoides spp.(Bud and LeafNematodes)<br/>14.1.19. Ipanema spp.(Dagger Nematodes)<br/>14.1.20. Longidorus spp.(Needle Nematodes)<br/>14.1.21. Trichodorus spp.(Stubby-root nematodes)<br/>14.2. Entomopathogenic Nematodes<br/>14.2.1. Steinernema<br/>14.2.2. Heterorhabditis<br/>15.TECHNIQUESUSEDTOCONTROLPLANTNEMATODES<br/>15.1. Soil Solarization<br/>15.2. Soil Fumigation with Methyl Bromide<br/>15.2.1. Nursery-Beds<br/>15.2.2. Potting Soil<br/>15.2.3. Phjrtotoxicity Test<br/> |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | General Books |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Full call number | Accession number | Date last seen | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Central Library, Sikkim University | Central Library, Sikkim University | General Book Section | 29/08/2016 | 571.999 RED/L | P31476 | 29/08/2016 | General Books |