Techniques in cancer research: a laboratory manual/ (Record no. 168520)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03651nam a2200229Ia 4500 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 8172361556 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CUS |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 571.978 |
| Item number | DEO/T |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Deo, M.G. |
| 245 #0 - TITLE STATEMENT | |
| Title | Techniques in cancer research: a laboratory manual/ |
| Statement of responsibility, etc. | M.G. Deo, R. Seshadri, R. Mulherkar and A.N. Bhisey |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | New Delhi: |
| Name of publisher, distributor, etc. | National Institute of Science Communication, |
| Date of publication, distribution, etc. | 1997. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | xvi, 379 p.; 23 cm. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc | Including index. |
| 505 ## - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Content <br/>Cell Biology -- Cell and Tissue Culture -- Primary cultures -- Cell transformation assays -- Chromosome preparation and banding techniques -- Autoradiography of cells and tissue sections -- Somatic cell hybridization --Cell cycle analysis -- Cell adhesion assay -- Flow cytometry and its use in biological systems -- Electron microscopy -- Biochemistry -- Cell and macromolecule separation -- Sub-cellular fractionation of liver tissue -- Radiolabelling of proteins and cells -- Protein purification -- Protein characterization -- Peptide mapping -- Amino acid analysis -- N- terminal amino acid sequencing of proteins -- Zymographic detection of proteases -- Plasma membrane studies with leetins -- Carbohydrate analysis -- Hormones and growth factors -- Molecular biology -- DNA extraction --Digestion of DNA with restriction endonucleases -- Southern hybridization analysis -- RNA extraction -- Northern blotting -- Polymerase Chain Reaction (PCR) -- Single strand conformation polymorphism (SSCP) analysis -- In-situ hybridization of mRNA -- Maintenance and storage of bacterial strains -- Transfromation of bacteria by plassmid DNA -- Small scale isolation of plasmid DNA -- Large scale perpartatiion of Plasmid DNA from bacterial cells -- Trandfecton of Mammalian cells with Plasmid DNA -- Cloning in M13 for nucleotide sequencing -- Nucleotide sequencing -- Oncogenes and suppressor genes -- Genetic Engineering -- Gene therapy -- Rapid retrovirus vector production in 293 cells -- Replication-Competent Retrovirus (RCR) detection -- VL30 detection by PCR -- Advenovirus vectors: CsCI purification -- Ademovirus vextors: Titration using plaque assay on 293v cells -- assay of B-galactosidase in cultured cells -- Bystander effect and anti-tumor activity -- Production of ransgenic mice by micro manipulation -- Virology -- Demonstration of viruses using nucleic acid probes -- Cultivation of human retroviruses -- Immunology -- Generation of polyclonal antibodies -- Purification of immunoglobulins -- Characterization of antibodies -- Immunoprecipitation -- Radioimmunoassay -- Enzyme linked immunsosorbent assay -- Cell ELISA -- Immunoflurorescence assay -- Immunodeficient staining techniques -- Hybridoma techniques -- Separation & isolation of blood cells -- T&B Lymphocyte surface markers -- Iymphocyte transformation -- Lymphokine activated killer (LAK) cells -- Functional assessment of effect or cells -- Bioassays for cytokines -- EBV transformed B-cell lines -- Establisment of T-cell clones -- Carcinogenesis -- Assessment of mutagenity and genotoxicity -- Assessment of carcinogenicity -- Analysis of tabacco and its products -- Assessment of human exposure of tobacco -- Measurement of exposure to alkylatic agents (Potential carcinogens) -- Measurement of detixifying enzymes -- Chemotherapy -- Screening of anti-cancer drugs -- Animal Sciences -- Laboratory animals-breeding, care and maintenance <br/>Appendix<br/>Index |
| 650 ## - SUBJECT | |
| Keyword | Cancer -- Research. |
| 650 ## - SUBJECT | |
| Keyword | Neoplasms. |
| 650 ## - SUBJECT | |
| Keyword | Research. |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Seshadri, R. |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mulherkar, R. |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Bhisey, A.N. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | General Books |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Full call number | Accession number | Date last seen | Date last checked out | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Central Library, Sikkim University | Central Library, Sikkim University | General Book Section | 29/08/2016 | 571.978 DEO/T | P23480 | 15/02/2023 | 07/02/2023 | General Books |