A comparative methods approach to the study of oocytes and embryos/
A comparative methods approach to the study of oocytes and embryos/
edited by Joel D. Richter
- New York: Oxford University Press, 1999.
- xv, 520 p. : ill. ; 24 cm.
- (Advances in molecular biology) .
PART ONE: METHODS FOR STUDYING MOUSE OOCYTES AND EMBRYOS --
1. Mouse Oocyte Maturation, Fertilization, and Preimplantation Development in Vitro --
2. Purification and Functional Analysis of Mouse Egg Zona Pellucida Glycoproteins --
3. Methods for Analysis of Cytoplasmic Signal Transduction in Mammalian Eggs and Embryos --
4. Intracellular Ion Measurements in Single Aggs and Embryos Using Ion-Sensitive Fluorophores --
5. Approahces o Study Translational Control in Mouse Oocytes --
6. Identification of Genes and Processes Guiding the Transition between the Mammalian Gamete and Embryo --
7. Approaches for the analysis of Genomic Imprinting in Oocytes and Early Mouse Embryos --
8. Gene Expression in Mouse Embryos: Use mRNA Differential Displays Differential Display --
9. Use of Differential Display to Identify Gene Expression Differences in Male and Female Mouse Genital Ridges --
PART TWO: METHODS FOR STUDYING XENOPUS OOCYTES AND EMBRYOS --
10. DNA Recombination and Repair in Xenopus Oocytes and Embryos --
11. DNA Replication and Chromatin Assembly Using Xenopus Eggs or Embryos --
12. Isolation and Characterization of Maked mRNPs from Xenopus Oocytes --
13. Complicationa and Problems in the Assay of Transcription in Xenopus Oocytes, Eggs, and Embryos --
14. Analysis of Localized RNAs in Xenopus Oocytes --
15. InSitu Hybridization Techniques with Xenopus Embryos --
16. Visualizing Endogenous and Exogenous Proteins in Xenopus laevis (and Other Organisms) --
17. Qualitative Analysis of Differential Gene Expression during Early Xenopus Embryogenesis by Whole-Mount In Situ Hybridization and RT-PCR --
18. Studying the Function of Maternal mRNAs in Xenopus Embryos: An Antisense Approach --
19. Testing the Cell Fate Commitment of Single Blastomeres in Xenopus laevis --
PART THREE: METHODS FOR STUDYING DROSOPHILA OOCYTES AND EMBRYOS --
20. Eggshell Assembly in Drosophila --
21. Cell Biological and Genetic Methods for Studying Drosophila Oogenesis --
22. Techniques for Analyzing Protein and RNA Distribution in Drosophila Ovaries and Embryos at Structural and Ultrastructural Resolution --
23. Analysis of RNA Distribution during Drosophila Oogenesis Using Fluorescent In Situ Hybridization --
24. Assessment of Poly(A) Tail Lengths on Specific mRNAs during Development --
25. Three-Dimensional, Time-Lapse Microscopy of Drosophila Embryogenesis --
26. Anatomical Techniques for Analysis of Nervous System Development in the Drosophila Embryo.
9780195115949
Mice
Frogs
Fruit-flies
571.861 / RIC/A
PART ONE: METHODS FOR STUDYING MOUSE OOCYTES AND EMBRYOS --
1. Mouse Oocyte Maturation, Fertilization, and Preimplantation Development in Vitro --
2. Purification and Functional Analysis of Mouse Egg Zona Pellucida Glycoproteins --
3. Methods for Analysis of Cytoplasmic Signal Transduction in Mammalian Eggs and Embryos --
4. Intracellular Ion Measurements in Single Aggs and Embryos Using Ion-Sensitive Fluorophores --
5. Approahces o Study Translational Control in Mouse Oocytes --
6. Identification of Genes and Processes Guiding the Transition between the Mammalian Gamete and Embryo --
7. Approaches for the analysis of Genomic Imprinting in Oocytes and Early Mouse Embryos --
8. Gene Expression in Mouse Embryos: Use mRNA Differential Displays Differential Display --
9. Use of Differential Display to Identify Gene Expression Differences in Male and Female Mouse Genital Ridges --
PART TWO: METHODS FOR STUDYING XENOPUS OOCYTES AND EMBRYOS --
10. DNA Recombination and Repair in Xenopus Oocytes and Embryos --
11. DNA Replication and Chromatin Assembly Using Xenopus Eggs or Embryos --
12. Isolation and Characterization of Maked mRNPs from Xenopus Oocytes --
13. Complicationa and Problems in the Assay of Transcription in Xenopus Oocytes, Eggs, and Embryos --
14. Analysis of Localized RNAs in Xenopus Oocytes --
15. InSitu Hybridization Techniques with Xenopus Embryos --
16. Visualizing Endogenous and Exogenous Proteins in Xenopus laevis (and Other Organisms) --
17. Qualitative Analysis of Differential Gene Expression during Early Xenopus Embryogenesis by Whole-Mount In Situ Hybridization and RT-PCR --
18. Studying the Function of Maternal mRNAs in Xenopus Embryos: An Antisense Approach --
19. Testing the Cell Fate Commitment of Single Blastomeres in Xenopus laevis --
PART THREE: METHODS FOR STUDYING DROSOPHILA OOCYTES AND EMBRYOS --
20. Eggshell Assembly in Drosophila --
21. Cell Biological and Genetic Methods for Studying Drosophila Oogenesis --
22. Techniques for Analyzing Protein and RNA Distribution in Drosophila Ovaries and Embryos at Structural and Ultrastructural Resolution --
23. Analysis of RNA Distribution during Drosophila Oogenesis Using Fluorescent In Situ Hybridization --
24. Assessment of Poly(A) Tail Lengths on Specific mRNAs during Development --
25. Three-Dimensional, Time-Lapse Microscopy of Drosophila Embryogenesis --
26. Anatomical Techniques for Analysis of Nervous System Development in the Drosophila Embryo.
9780195115949
Mice
Frogs
Fruit-flies
571.861 / RIC/A