Contents: Cover -- Half Title -- Title Page -- Copyright Page -- Overview and scheduling of laboratory exercises -- Editors -- Contributors -- Acknowledgments -- Table of Contents -- Part 1: Introduction -- Chapter one: Introduction to plant tissue culture -- Part II: History of plant tissue culture -- Chapter two: History of plant tissue and cell culture -- Part Ill: Supporting methodologies -- Chapter three: Getting started with tissue culture -- media preparation, sterile technique, and laboratory equipment -- Chapter four: Nutrition of callus cultures -- Chapter five: Histological techniques -- Chapter six: Photographic methods for plant cell and tissue culture -- Chapter seven: Statistical analysis of plant tissue culture data -- Part IV: Propagation techniques -- Chapter eight: Propagation from preexisting meristems -- Chapter nine: Micropropagation of Syngonium by shoot culture -- Chapter ten: Micropropagation of Dieffenbachia -- Chapter eleven: Micropropagation of potato by node culture and microtuber production -- Chapter twelve: Micropropagation of lilacs -- Chapter thirteen: Micropropagation and in vitro flowering of rose -- Chapter fourteen: Organogenesis -- Chapter fifteen: Direct shoot organogenesis from leaf explants of chrysanthemum -- Chapter sixteen: Shoot organogenesis from cotyledon explants of watermelon -- Chapter seventeen: Direct and indirect shoot organogenesis from leaves of Torenia fournieri -- Chapter eighteen: Shoot organogenesis from petunia leaves -- Chapter nineteen: Nonzygotic embryogenesis -- Chapter twenty: Embryogenic callus and suspension cultures from leaves of orchardgrass -- Chapter twenty-one: Somatic embryogenesis from seeds of melon -- Chapter twenty-two: Somatic embryogenesis from mature peanut seed -- Chapter twenty-three: Direct somatic embryogenesis from leaves and flower receptacles of cineraria.
Contents: Chapter twenty-four: Somatic embryogenesis from immature seeds of yellow poplar -- Chapter twenty-five: Induction of somatic embryogenesis in conifers -- Part V: Crop improvement techniques -- Chapter twenty-six: Use of protoplasts for plant improvement -- Chapter twenty-seven: Isolation, culture, and fusion of tobacco and potato protoplasts -- Chapter twenty-eight: Isolation of protoplasts from leaves of chrysanthemum and orchardgrass -- Chapter twenty-nine: Haploid cultures -- Chapter thirty: Production of haploid tobacco plants using anther culture -- Chapter thirty-one: Transformation -- Chapter thirty-two: Transformation of tobacco and carrot using Agrobacterium tumefaciens and expression of the p-glucuronidase (GUS) reporter gene -- Chapter thirty-three: Transformation of chrysanthemum leaf explants using Agrobacterium tumefaciens -- Chapter thirty-four: Construction and use of a simple gene gun for particle bombardment -- Chapter thirty-five: Germplasm preservation -- Chapter thirty-six: Vitrification as a method to cryopreserve shoot tips -- Chapter thirty-seven: Secondary product expression in vitro -- Chapter thirty-eight: Pigment production in Ajuga cell cultures -- Chapter thirty-nine: In vitro plant pathology -- Part VI: Special topics -- Chapter forty: Variation in tissue culture -- Chapter forty-one: Short-circuiting the fern life cycle: apospory in Ceratopteris richardii -- Chapter forty-two: Commercial laboratory production -- Chapter forty-three: Indexing for plant pathogens -- Chapter forty-four: Culture indexing for bacterial and fungal contaminants -- Part VII: Appendix -- Appendix I: List of some suppliers, addresses, and toll-free telephone numbers -- Part VIII: Index -- Index.